LOCUS       sequence-360744-        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SPLINTR backbone vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Fennell KA, Vassiliadis D, Lam EYN, Martelotto LG, Balic JJ, 
            Hollizeck S, Weber TS, Semple T, Wang Q, Miles DC, MacPherson L, 
            Chan YC, Guirguis AA, Kats LM, Wong ES, Dawson SJ, Naik SH, Dawson 
            MA
  TITLE     Non-genetic determinants of malignant clonal fitness at single-cell 
            resolution.
  JOURNAL   Nature. 2022 Jan;601(7891):125-131. doi: 10.1038/s41586-021-04206-7.
            Epub 2021 Dec 8.
  PUBMED    34880496
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-04206-7"; journalName: "Nature"; date: 
            "2022-01"; volume: "601"; issue: "7891"; pages: "125-131"
COMMENT     Sequence Label: SPLINTR BFP v1 backbone
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     795..815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             858..1038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1085..1210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1703..1936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2121..2165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2463..2580
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2705..3883
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2936..3874
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3831..3851
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3890..3899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3896..3898,3899..3901,3902..4615)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(3941..3962)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4640..5228
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4693..4713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5111..5122)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5231..5247)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5232..5248)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5440..5620
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5646..5663)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5652..5876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5922..6350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6009..6028)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6219..6240
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6359..6379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6364..6693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6544..6679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6606..6625
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6741..6788
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6807..7181
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7311..7432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7348..7367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7402..7421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7481..7497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7481..7497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7494..7516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7505..7521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7529..7535,7536..7553,7554..7559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7712..7729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7883..8471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7963..7982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8642..9433,9434..9502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9265..9284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9503..9607)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1021 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1081 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1141 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1201 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1261 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1321 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1381 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1441 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1501 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1561 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1621 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1681 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1741 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1801 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1861 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1921 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1981 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2041 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2101 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2161 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2221 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2281 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2341 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2401 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     2461 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2521 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2581 cgggtttatt acagggacag cagagatcca gtttggttaa ttaatgcaaa gatggataaa
     2641 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     2701 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2761 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     2821 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2881 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2941 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     3001 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     3061 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     3121 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     3181 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     3241 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     3301 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     3361 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     3421 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     3481 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     3541 aaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     3601 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     3661 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     3721 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     3781 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     3841 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatggt
     3901 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3961 cgtaaacggc cacaagttca gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa
     4021 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4081 gaccaccctg agccacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     4141 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4201 ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4261 ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4321 ggagtacaac ttcaacagcc acaacatcta tatcatggcc gtcaagcaga agaacggcat
     4381 caaggtgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca
     4441 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca gccactacct
     4501 gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4561 ggagttccgc accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt
     4621 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5041 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5161 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5221 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     5281 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5341 gggttttcca gtcacacctc aggtaccaat gtcgtctcgg atcctccgag tgcgtctcgc
     5401 tagcgctcta gtctgagtct agactgtccg gactgtactg ggtctctctg gttagaccag
     5461 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5521 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5581 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     5641 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5701 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5761 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5821 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5881 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5941 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6001 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6061 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6121 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6181 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6241 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6301 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6361 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6421 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6481 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6601 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6661 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6721 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     6781 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     6841 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     6901 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     6961 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     7021 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     7081 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     7141 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     7201 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7261 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7441 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9601 ttccgcgcac at
//
