LOCUS       sequence-360747-        9667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SPLINTR backbone vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Fennell KA, Vassiliadis D, Lam EYN, Martelotto LG, Balic JJ, 
            Hollizeck S, Weber TS, Semple T, Wang Q, Miles DC, MacPherson L, 
            Chan YC, Guirguis AA, Kats LM, Wong ES, Dawson SJ, Naik SH, Dawson 
            MA
  TITLE     Non-genetic determinants of malignant clonal fitness at single-cell 
            resolution.
  JOURNAL   Nature. 2022 Jan;601(7891):125-131. doi: 10.1038/s41586-021-04206-7.
            Epub 2021 Dec 8.
  PUBMED    34880496
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-04206-7"; journalName: "Nature"; date: 
            "2022-01"; volume: "601"; issue: "7891"; pages: "125-131"
COMMENT     Sequence Label: SPLINTR mCHERRY v1 backbone
FEATURES             Location/Qualifiers
     source          1..9667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..42)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        214..593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        595..793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     748..768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             811..991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1038..1163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1656..1889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2074..2118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2416..2533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2658..3836
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2889..3827
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3784..3804
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3853..3906
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3907..4617
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4052..4070)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4320..4339
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4648..5236
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4701..4721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5119..5130)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5239..5255)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5240..5256)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5448..5628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5654..5671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5660..5884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5930..6358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6017..6036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6227..6248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6367..6387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6372..6701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6552..6687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6614..6633
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6749..6796
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6815..7189
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7319..7440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7356..7375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7410..7429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7489..7505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7489..7505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7502..7524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7513..7529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7537..7543,7544..7561,7562..7567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7582..7603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7720..7737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7891..8479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7971..7990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8650..9441,9442..9510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9273..9292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9511..9615)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
       61 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
      121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      781 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
      841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      961 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1021 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1081 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1141 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1201 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1261 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1321 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1381 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1441 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1501 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1561 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1621 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1681 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1741 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1801 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1861 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1921 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1981 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     2041 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     2101 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2161 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2221 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2281 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2341 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2401 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2461 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2521 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaatgc
     2581 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     2641 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2701 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2761 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2821 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2881 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2941 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     3001 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     3061 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3121 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3181 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3241 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3301 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3361 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3421 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3481 ggagctcaaa atgaaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3541 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3601 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3661 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3721 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3781 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta
     3841 cctagcggcg gtgagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     3901 ggcccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     3961 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     4021 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     4081 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     4141 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     4201 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     4261 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     4321 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     4381 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     4441 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     4501 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4561 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagtct
     4621 agagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     4681 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4741 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4801 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4861 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4921 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4981 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5041 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5101 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5161 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5221 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     5281 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5341 gaggaggtgg gttttccagt cacacctcag gtaccaatgt cgtctcggat cctccgagtg
     5401 cgtctcgcta gcgctctagt ctgagtctag actgtccgga ctgtactggg tctctctggt
     5461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     5641 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5701 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5761 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5821 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5881 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5941 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6001 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6061 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6121 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6181 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6241 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6301 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6361 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6421 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6541 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6601 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6661 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6721 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     6781 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     6841 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     6901 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     6961 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     7021 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     7081 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     7141 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     7201 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7261 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7441 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9661 tctcccg
//
