LOCUS       sequence-360751-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(240..259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      689..1277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1178..1197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1404..1409,1410..1426,1427..1432)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1480..2604,1..66)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1832..1851)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
       61 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
      121 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta
      181 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc
      241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      361 aagctggtct cgcaggttgt agcgattgct agtaacaatg gtggcaaaca ggctcttgaa
      421 accgtacagc gcctactgcc agttctctgt caagcccacg gtctgactcc ggagcaagtt
      481 gtagcgattg ctagtaatat tggtggcaaa caggctcttg agacggttca gcgcctcctt
      541 ccagttctct gtcaagccca cggactcacc ccagatcaag tggttgagac catatctggt
      601 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
      661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1321 ggccttttgc tcacatgttc tttcctgcgt tatccacaga atcaggggat aacgcaggaa
     1381 agaacatgaa ttaattctca tgtttgacag cttatcatcg attagcttta atgcggtagt
     1441 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
     1501 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
     1561 ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg
     1621 ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
     1681 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     1741 gcgatcatgg cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggccggc
     1801 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     1861 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     1921 cccgtggccg ggggactgtt gggcgccatc tccttacatg caccattcct tgcggcggcg
     1981 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     2041 gagcgccgac ccatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     2101 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     2161 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     2221 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     2281 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     2341 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     2401 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     2461 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     2521 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     2581 gggttggcat ggattgtagg cgcc
//
