LOCUS       sequence-360768-        7445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR 
            modified to bring the viral genome below the optimal AAV packaging 
            limit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7445)
  TITLE     Ploski Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)
FEATURES             Location/Qualifiers
     source          1..7445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   10..139
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        158..1446
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3270..3279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3276..3986
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3421..3439)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3689..3708
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4011..4599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4064..4084)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4482..4493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4659..4678)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4717..4857
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4717..4846
                     /label=AAV2 ITR
     rep_origin      4932..5387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5019..5038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5229..5250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5404..5423)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5523..5545
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5669..5773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5774..5842,5843..6634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5992..6011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6805..7393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7294..7313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcacatgtc ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg
       61 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac
      121 tccatcacta ggggttcctg cggccgcacg cgtttaacat tatggcctta ggtcacttca
      181 tctccatggg gttcttcttc tgattttcta gaaaatgaga tgggggtgca gagagcttcc
      241 tcagtgacct gcccagggtc acatcagaaa tgtcagagct agaacttgaa ctcagattac
      301 taatcttaaa ttccatgcct tgggggcatg caagtacgat atacagaagg agtgaactca
      361 ttagggcaga tgaccaatga gtttaggaaa gaagagtcca gggcagggta catctacacc
      421 acccgcccag ccctgggtga gtccagccac gttcacctca ttatagttgc ctctctccag
      481 tcctaccttg acgggaagca caagcagaaa ctgggacagg agccccagga gaccaaatct
      541 tcatggtccc tctgggagga tgggtgggga gagctgtggc agaggcctca ggaggggccc
      601 tgctgctcag tggtgacaga taggggtgag aaagcagaca gagtcattcc gtcagcattc
      661 tgggtctgtt tggtacttct tctcacgcta aggtggcggt gtgatatgca caatggctaa
      721 aaagcaggga gagctggaaa gaaacaagga cagagacaga ggccaagtca accagaccaa
      781 ttcccagagg aagcaaagaa accattacag agactacaag ggggaaggga aggagagatg
      841 aattagcttc ccctgtaaac cttagaaccc agctgttgcc agggcaacgg ggcaatacct
      901 gtctcttcag aggagatgaa gttgccaggg taactacatc ctgtctttct caaggaccat
      961 cccagaatgt ggcacccact agccgttacc atagcaactg cctctttgcc ccacttaatc
     1021 ccatcccgtc tgttaaaagg gccctatagt tggaggtggg ggaggtagga agagcgatga
     1081 tcacttgtgg actaagtttg ttcgcatccc cttctccaac cccctcagta catcaccctg
     1141 ggggaacagg gtccacttgc tcctgggccc acacagtcct gcagtattgt gtatataagg
     1201 ccagggcaaa gaggagcagg ttttaaagtg aaaggcaggc aggtgttggg gaggcagtta
     1261 ccggggcaac gggaacaggg cgtttcggag gtggttgcca tggggacctg gatgctgacg
     1321 aaggctcgcg aggctgtgag cagccacagt gccctgctca gaagccccaa gctcgtcagt
     1381 caagccggtt ctccgtttgc actcaggagc acgggcaggc gagtggcccc tagttctggg
     1441 ggcagctcta gagcggtacc ggatcctcta gagtcgacgc caccatgacc ttgcacaata
     1501 acagtacaac ctcgcctttg tttccaaaca tcagctcctc ctggatacac agcccctccg
     1561 atgcagggct gcccccggga accgtcactc atttcggcag ctacaatgtt tctcgagcag
     1621 ctggcaattt ctcctctcca gacggtacca ccgatgaccc tctgggaggt cataccgtct
     1681 ggcaagtggt cttcatcgct ttcttaacgg gcatcctggc cttggtgacc atcatcggca
     1741 acatcctggt aattgtgtca tttaaggtca acaagcagct gaagacggtc aacaactact
     1801 tcctcttaag cctggcctgt gccgatctga ttatcggggt catttcaatg aatctgttta
     1861 cgacctacat catcatgaat cgatgggcct tagggaactt ggcctgtgac ctctggcttg
     1921 ccattgactg cgtagccagc aatgcctctg ttatgaatct tctggtcatc agctttgaca
     1981 gatacttttc catcacgagg ccgctcacgt accgagccaa acgaacaaca aagagagccg
     2041 gtgtgatgat cggtctggct tgggtcatct cctttgtcct ttgggctcct gccatcttgt
     2101 tctggcaata ctttgttgga aagagaactg tgcctccggg agagtgcttc attcagttcc
     2161 tcagtgagcc caccattact tttggcacag ccatcgctgg tttttatatg cctgtcacca
     2221 ttatgactat tttatactgg aggatctata aggaaactga aaagcgtacc aaagagcttg
     2281 ctggcctgca agcctctggg acagaggcag agacagaaaa ctttgtccac cccacgggca
     2341 gttctcgaag ctgcagcagt tacgaacttc aacagcaaag catgaaacgc tccaacagga
     2401 ggaagtatgg ccgctgccac ttctggttca caaccaagag ctggaaaccc agctccgagc
     2461 agatggacca agaccacagc agcagtgaca gttggaacaa caatgatgct gctgcctccc
     2521 tggagaactc cgcctcctcc gacgaggagg acattggctc cgagacgaga gccatctact
     2581 ccatcgtgct caagcttccg ggtcacagca ccatcctcaa ctccaccaag ttaccctcat
     2641 cggacaacct gcaggtgcct gaggaggagc tggggatggt ggacttggag aggaaagccg
     2701 acaagctgca ggcccagaag agcgtggacg atggaggcag ttttccaaaa agcttctcca
     2761 agcttcccat ccagctagag tcagccgtgg acacagctaa gacttctgac gtcaactcct
     2821 cagtgggtaa gagcacggcc actctacctc tgtccttcaa ggaagccact ctggccaaga
     2881 ggtttgctct gaagaccaga agtcagatca ctaagcggaa aaggatgtcc ctggtcaagg
     2941 agaagaaagc ggcccagacc ctcagtgcga tcttgcttgc cttcatcatc acttggaccc
     3001 catacaacat catggttctg gtgaacacct tttgtgacag ctgcataccc aaaacctttt
     3061 ggaatctggg ctactggctg tgctacatca acagcaccgt gaaccccgtg tgctatgctc
     3121 tgtgcaacaa aacattcaga accactttca agatgctgct gctgtgccag tgtgacaaaa
     3181 aaaagaggcg caagcagcag taccagcaga gacagtcggt catttttcac aagcgcgcac
     3241 ccgagcaggc cttgaaggat cccccggtcg ccaccatggt gagcaagggc gaggaggata
     3301 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     3361 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3421 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     3481 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     3541 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     3601 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     3661 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3721 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3781 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3841 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3901 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3961 gcggcatgga cgagctgtac aagtaagaat tcgatatcaa gcttatcgat aatcaacctc
     4021 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4081 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4141 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4201 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4261 ttgccaccac ctgtcagctc ctttccggaa ctttcgcttt ccccctccct attgccacgg
     4321 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4381 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg
     4441 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4501 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4561 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg
     4621 agagatcttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4681 aaataaagca tttttttcac tggaccgagc ggccgcagga acccctagtg atggagttgg
     4741 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     4801 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     4861 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4921 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4981 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     5041 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5101 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     5161 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5221 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     5281 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     5341 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     5401 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5461 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5521 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5581 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5641 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5701 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5761 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5821 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5881 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5941 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6001 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6061 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6121 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6181 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6241 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6301 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6361 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6421 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6481 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6541 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6601 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6661 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6721 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6781 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6841 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6901 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6961 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7021 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7081 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7141 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7201 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7261 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7321 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7381 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7441 ttttg
//
