LOCUS       sequence-36083-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     Koloteva-Levine et al
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTH342-SUP45(276-437)-GalBD
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..68
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      594..641
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      668..855
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(723..747)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(879..895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(879..895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtatacgact cactataggg cgagccgcca tcatggagga gcagaagctg atctcagagg
       61 aggacctgca tatggccatg gaggtcaagt atgttcaaga aaagaaatta ttggaggcat
      121 attttgacga aatttcccag gacactggta aattctgtta tggtatagat gatactttaa
      181 aggcattgga tttaggtgca gtcgaaaaat taattgtttt cgaaaatttg gaaactatca
      241 gatatacatt taaagatgcc gaggataatg aggttataaa attcgctgaa ccagaagcca
      301 aggacaagtc gtttgctatt gacaaagcta ccggccaaga aatggacgtt gtctccgaag
      361 aacctttaat tgaatggcta gcagctaact acaaaaactt cggtgctacc ttggaattca
      421 tcacagacaa atcttcagaa ggtgcccaat ttgtcacagg ttttggtggt attggtgcca
      481 tgctgcgtta caaagttaat tttgaacaac tagttgatga atctgaggat gaatattatg
      541 acgaagatga aggatccgac tatgatttca tttaanntgc agcggccgca taactagcat
      601 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gcgcgcttgc agccaagcta
      661 attccgggcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
      721 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
      781 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
      841 ccacacctct accggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtg
//
