LOCUS       sequence-360860-        6671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector containing a yeast integration cassette for RPL18B-driven 
            expression of ARC35-Link-Scarlet(rfp), a marker for the actin 
            cytoskeleton.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Bean BDM, Whiteway M, Martin VJJ
  TITLE     The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and 
            Overexpression CRISPR-Cas9 Toolkit.
  JOURNAL   G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154.
  PUBMED    35708612
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154."
COMMENT     Sequence Label: pBBK71
            pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-S...
FEATURES             Location/Qualifiers
     source          1..6671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1069..1657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1558..1577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgccaatgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgcccc ctatttatcg gcattgagat tgtaaaggaa
     1921 gtaaaacgca tttttgcaga tctgttctct tatgtatttt taatcgtcct tgtatggaag
     1981 tatcaaaggg gacgttcttc acctccttgg aaaacgcaaa ctttcttttt gcctatcaca
     2041 gtatcttatc gagctaacta ttttcgacac acatgaaaaa gcagaaatat taacgaaaaa
     2101 gaaaagaaag accatgtcat gtacgggcaa tcagaatatg caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaatcccac agtgagcaac
     2281 ttgacaaacc aacagatata ggctgtgtct taaaatatac catccaatac cttgatgaac
     2341 ttttcaaaga tgactaaaat aagtgaaatt tcaacattaa cttcgagctg taaacacagt
     2401 ggtttctttg cataaacacc atcagcctca agtcgtcaag taaagatttc gtgttcatgc
     2461 agatagataa caatctatat gttgataatt agcgttgcct catcaatgcg tacacgaaga
     2521 atatactaaa aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc
     2581 tagtaaagcg tattacaaat gaaaccaaga ttcagattgc gatccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatctatg ctacacttac
     3541 aaccacaaaa ccttctcatc caaaagacac tgaatgaagc gatagaggca cttcgcaagg
     3601 gttcgccgtt gaccatggac agaattgttt ccgatttcga ctataccacc taccacattt
     3661 caaacacagc agaagataag tccattctgt tgttgagtgt taagaccaaa gcttgggtaa
     3721 gtgtctctga gtgccaattg gacggttctt tgacgttact gaagtttctg gccgaccact
     3781 actcctcttt gggtggggtg actatcccaa gtgaagtgga gcccggctat gactacactc
     3841 ttcagattac gttggccgaa ttggttcaag aatcgatttt acaactgtct gttttgaaga
     3901 ccattatatt gagttttccc ttcgaattgg ccatttcgaa gtttattgaa ctaagccagc
     3961 agcaaccggc acctgtcgaa gcagaaatta ctggcggtga ggttgctgcc aatggagaca
     4021 acactttgtt cacaatcaag tatcgtgacg aggagaacat cttcatcaaa ccttccaatg
     4081 acagagttac cataatcttc gagactattt ttcaagacga aacagataaa atttttggca
     4141 aggtcttttt acaagaattc gtggatgcac gtaagagaaa ccgtcaaatc caatccgctc
     4201 cacaagtttt atactcacat gaaccaccat tggagttgaa gagactatat caaccaccta
     4261 aagttgcaga gcaaagtaga agatttatca cgtttgttct tttcccacgt catttccaga
     4321 caaaagagtt gcaattccat tcaatttgcc agttaacatt gtttagaaac tacttccatt
     4381 atcacataaa gtgctctaaa gcctacatgc actccagaat gagattcaga gtggactcgt
     4441 tcatcaaagt cttaaataga gccaaggtgg acgaagatga tgaaaatgat gaattgagtg
     4501 cagaaggcag acaacaggca agaagaacct tcaccggtag aaagattgtc tacggatccg
     4561 gtccttctgg cccaactggc atggttagta aaggtgaagc tgttataaaa gaattcatga
     4621 ggtttaaagt tcatatggaa ggttcaatga atggtcatga atttgaaatt gaaggtgaag
     4681 gtgaaggtag accatatgaa ggtacacaaa ctgctaaatt gaaagttact aaaggtggtc
     4741 cattaccatt ttcttgggat attttgtctc cacaattcat gtatggttct agagctttta
     4801 ttaagcatcc agctgatatt ccagattatt ataaacaatc ttttcctgaa ggttttaaat
     4861 gggaaagagt tatgaatttc gaagatggtg gtgctgttac tgttactcaa gatacttctt
     4921 tggaagatgg tactttaatc tataaagtta aattgagagg tactaatttt ccaccagatg
     4981 gtccagttat gcaaaagaaa actatgggtt gggaagcatc tactgaaaga ttgtatccag
     5041 aagatggtgt tttgaaagga gacattaaga tggctttgag attgaaagat ggtggtcggt
     5101 acctggccga cttcaagacc acctataaag ctaaaaaacc agttcaaatg ccaggtgcat
     5161 ataatgttga tagaaagtta gatataacgt cgcataacga ggactatact gtagttgaac
     5221 aatatgaacg tagtgaaggt agacatagta ccggaggaat ggatgaattg tataaatagc
     5281 tcgagtggcg ccaattggtg cggcaattga taataacgaa aatgtctttt aatgatctgg
     5341 gtataatgag gaattttccg aacgttttta ctttatatat atatatacat gtaacatata
     5401 ttctatacgc tatagagaaa ggaaattttt caattaaaaa aaaaatagag aaagagtttc
     5461 acttcttgat tatcgctaac actaatggtt gaagtactgc tactttaatt ttatgctgcc
     5521 aatgagacga cggggtcatc acggctcatc atgcgccaaa caaatgtgtg caatacacgc
     5581 tcggatgact gcatgatgac cgcactgact ggggacagca gatccaccta agcctgtgag
     5641 agaagcagac acccgacaga tcaaggcagt taactagtgc actgcagtac aaatcgtccc
     5701 caacaaaagt gggctctcaa aattcatcac atttaaatgc atataggaag agcaacagtt
     5761 ggtttgcatc tgatgttcct taaagatttc gacataatgt gcgaagtaga tcccttcagc
     5821 actgtacttg tttttgcgac tagattgtaa atcattcttt atttaatctc tttctttaac
     5881 tactgcttaa agtataattt ggtccgtagt ttaataacta tactaagcga acgctctctc
     5941 gaaatttttc ttaacgcgtc cttgtactgc gtctaacgct tttgccactt ggatttctat
     6001 tataggaaat agtctcactt actgggcgac gaattttcgc gttttgatga agcacaggaa
     6061 gaatttcttt atgctcagaa gtttgacagc aagcaagttc atcattcgaa ctagccttat
     6121 tgttttagtt cagtgacagc gaactgccgt actcgatgct ttatttctca cggtagagcg
     6181 gaagaacaga tagatcccta catattgcta ccacttctat tacacaatag tttcaatagc
     6241 ttgcagcgta gctaaactct aaaatttatc taaatcactc atataaaccg aacccttccc
     6301 cttccgctta tagtacagct gatgctcctt cactatttta acatgtggaa ttcttgaaag
     6361 aatgaaatcg ccatgccaag ccatcacacg gtcttttatg caattgattg accgcctgca
     6421 acacataggc agtaaaattt ttacaagcag tagcagaaca ggccacacaa tcgcaagtga
     6481 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
     6541 ccggctggtc gctaatcgtt gagtgccgag cggccgcgat tatcaaaaag gatcttcacc
     6601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6661 tggtctgaca g
//
