LOCUS       sequence-360871-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(214..219,220..236,237..242))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(369..957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(449..468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1365..1381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1365..1381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1386..1405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1386..1404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1579..2603,1..166))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2398..2417
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
       61 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
      121 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
      181 tgcgttagca atttaactgt gataaactac cgcattaaag ctaatcgatg ataagctgtc
      241 aaacatgaga attaattcat gttctttcct gcgttatccc ctgattctgt ggataacgca
      301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      541 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      721 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      781 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta actcacgtta
     1021 agggattttg gtcatgggtg gctcgaccag atatggtctc aggctatcgc tacaacttga
     1081 tctggggtga gtccgtgggc ttgacagaga actggaagga ggcgctgaac cgtctcaaga
     1141 gcctgtttgc caccaatatt actagcaatc gctacaactt gctccggagt cagaccgtgg
     1201 gcttgacaga gaactggcag taggcgctgt acggtttcaa gagcctgttt gccaccattg
     1261 ttactagcaa tccgagacca gctttggtct agaggaggca tgatgatata tttttatctt
     1321 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga
     1381 ccatgtaata cgactcacta taggggatat tttattttct cccaatcagg cttgatcccc
     1441 agtaagtcaa aaaatagctc gacatactgt tcttccccga tcaagggttg gtttgcgcat
     1501 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     1561 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     1621 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
     1681 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt
     1741 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     1801 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     1861 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat
     1921 gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc
     1981 ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct
     2041 ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag
     2101 ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg
     2161 gaaggagctg actgggttga aggctctcaa gggcatgggt cggcgctctc ccttatgcga
     2221 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
     2281 gaatggtgca tgtaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     2341 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     2401 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     2461 gcgtccggcg tagagaatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt
     2521 ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc
     2581 gagaacgggt gcgcatagaa att
//
