LOCUS       sequence-360872-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     605..621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     605..621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(819..2009)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1638..1657
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2057..2062,2063..2079,2080..2085))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2212..2603,1..197))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2292..2311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
       61 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      241 acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgaccag atatggtctc
      301 aacttgcaat cgctacaact tgatctgggg tgagtccgtg ggcttgacag agaactggaa
      361 ggaggcgctg aaccgtctca agagcctgtt tgccaccaat attactagca atcgctacaa
      421 cttgctccgg agtcagaccg tgggcttgac agagaactgg cagtaggcgc tgtacggttt
      481 caagagcctg tttgccacca ttgttactgg ctcgagacca gctttggtct agaggaggca
      541 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag
      601 ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat tttattttct
      661 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
      721 tcaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg
      781 agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc
      841 catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat
      901 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
      961 atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     1021 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     1081 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     1141 cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     1201 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     1261 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     1321 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     1381 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatgggt
     1441 cggcgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     1501 ttgagcaccg ccgccgcaag gaatggtgca tgtaaggaga tggcgcccaa cagtcccccg
     1561 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     1621 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     1681 ccggtgatgc cggccacgat gcgtccggcg tagagaatcc acaggacggg tgtggtcgcc
     1741 atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca
     1801 aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc
     1861 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg
     1921 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
     1981 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
     2041 tgtgataaac taccgcatta aagctaatcg atgataagct gtcaaacatg agaattaatt
     2101 catgttcttt cctgcgttat cccctgattc tgtggataac gcaggaaaga acatgtgagc
     2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2581 tagcagagcg aggtatgtag gcg
//
