LOCUS       sequence-360873-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..19)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(24..40)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(24..40)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      445..1033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     934..953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1160..1165,1166..1182,1183..1188)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1236..2426
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1588..1607)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(join(2600,1..19))
                     /label=T7
                     /note="T7 promoter, forward primer"
ORIGIN
        1 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
       61 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      121 aagctggtct cgaagtaaca atggtggcaa acaggctctt gaaaccgtac agcgcctact
      181 gccagttctc tgtcaagccc acggtctgac tccggagcaa gttgtagcga ttgctagtaa
      241 tattggtggc aaacaggctc ttgagacggt tcagcgcctc cttccagttc tctgtcaagc
      301 ccacggactc accccagatc aagttgtagc gattgcttcc tgagaccata tctggtcgag
      361 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
      421 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      481 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      541 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      601 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      661 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      721 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      781 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      841 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      901 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      961 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1021 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1081 ttttgctcac atgttctttc ctgcgttatc cacagaatca ggggataacg caggaaagaa
     1141 catgaattaa ttctcatgtt tgacagctta tcatcgatta gctttaatgc ggtagtttat
     1201 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
     1261 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
     1321 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
     1381 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
     1441 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
     1501 tcatggcgac cacacccgtc ctgtggattc tctacgccgg acgcatcgtg gccggcatca
     1561 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     1621 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     1681 tggccggggg actgttgggc gccatctcct tacatgcacc attccttgcg gcggcggtgc
     1741 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     1801 gccgacccat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     1861 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     1921 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     1981 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     2041 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     2101 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     2161 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     2221 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     2281 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     2341 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     2401 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     2461 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     2521 ttgatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     2581 tgggagaaaa taaaatatcc
//
