LOCUS       sequence-360877-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     784..800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     784..800
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     805..824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        805..823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(998..2188)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1817..1836
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2236..2241,2242..2258,2259..2264))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2391..2600,1..379))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2471..2490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
       61 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      421 gaacgacgcg taactcacgt taagggattt tggtcatggg tggctcgacc agatatggtc
      481 tcatgacgca atcgctacaa cttgatctgg ggtgagtccg tgggcttgac agagaactgg
      541 aaggaggcgc tgaaccgtct caagagcctg tttgccacca atattactag caatcgctac
      601 aacttgctcc ggagtcagac cgtgggcttg acagagaact ggcagtaggc gctgtacggt
      661 ttcaagagcc tgtttgccac cattgttaga gcgagaccag ctttggtcta gaggaggcat
      721 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
      781 tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatt ttattttctc
      841 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      901 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
      961 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     1021 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     1081 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa
     1141 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     1201 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     1261 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     1321 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     1381 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     1441 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     1501 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     1561 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatgggtc
     1621 ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     1681 tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat ggcgcccaac agtcccccgg
     1741 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     1801 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     1861 cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt gtggtcgcca
     1921 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     1981 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     2041 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     2101 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     2161 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     2221 gtgataaact accgcattaa agctaatcga tgataagctg tcaaacatga gaattaattc
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacg caggaaagaa catgtgagca
     2341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2581 tctcatagct cacgctgtag
//
