LOCUS       sequence-360881-        2501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pG7
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(84..672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(164..183)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     978..994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     978..994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     999..1018
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        999..1017
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1192..2382)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2011..2030
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2430..2435,2436..2452,2453..2458))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
       61 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      121 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      181 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      241 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      301 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      361 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      421 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      481 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      541 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      601 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      661 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac
      721 gcgtaactca cgttaaggga ttttggtcat gggtggctcg accagatatg gtctcaactt
      781 gcaatcgcta caacttgctc cggagtcaga ccgtgggctt gacagagaac tggcagtagg
      841 cgctgtacgg tttcaagagc ctgtttgcca ccattgttac tggctcgaga ccagctttgg
      901 tctagaggag gcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      961 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
     1021 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     1081 tgttcttccc cgatcaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
     1141 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
     1201 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
     1261 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
     1321 gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
     1381 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
     1441 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
     1501 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc
     1561 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
     1621 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
     1681 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     1741 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     1801 caagggcatg ggtcggcgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1861 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgtaagg agatggcgcc
     1921 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1981 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     2041 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagaa tccacaggac
     2101 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac
     2161 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat
     2221 caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat
     2281 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
     2341 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
     2401 ttagcaattt aactgtgata aactaccgca ttaaagctaa tcgatgataa gctgtcaaac
     2461 atgagaatta attcatgttc tttcctgcgt tatcccctga t
//
