LOCUS       sequence-360883-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pG8
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..332
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(539..544,545..561,562..567)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             615..1805
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(967..986)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1979..1998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1980..1998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2003..2019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2322..2498,1..412)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
       61 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      181 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      481 tgcgttatcc acagaatcag gggataacgc aggaaagaac atgaattaat tctcatgttt
      541 gacagcttat catcgattag ctttaatgcg gtagtttatc acagttaaat tgctaacgca
      601 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc
      661 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg
      721 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
      781 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc
      841 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc
      901 tgtggattct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      961 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     1021 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     1081 ccatctcctt acatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     1141 tgggctgctt cctaatgcag gagtcgcata agggagagcg ccgacccatg cccttgagag
     1201 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     1261 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     1321 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     1381 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     1441 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     1501 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     1561 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     1621 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg ctgatcgtca
     1681 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg
     1741 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     1801 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     1861 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tgatcgggga agaacagtat
     1921 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatatccc
     1981 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2041 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccaa
     2101 agctggtctc gaagtaacaa tggtggcaaa caggctcttg aaaccgtaca gcgcctactg
     2161 ccagttctct gtcaagccca cggtctgact ccggagcaag ttgtagcgat tgcttcctga
     2221 gaccatatct ggtcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     2281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgttc ttctagtgta gccgtagtta ggccacca
//
