LOCUS       sequence-360884-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..1216
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(378..397)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1390..1409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1391..1409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1414..1430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1414..1430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      1843..2431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2332..2351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2558..2563,2564..2580,2581..2586)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
       61 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      121 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      181 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      241 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      301 cacacccgtc ctgtggattc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      361 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      421 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      481 actgttgggc gccatctcct tacatgcacc attccttgcg gcggcggtgc tcaacggcct
      541 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gccgacccat
      601 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      661 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      721 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      781 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      841 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      901 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
      961 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1021 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     1081 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1141 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1201 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1261 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg
     1321 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     1381 taaaatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1441 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     1501 ctctagacca aagctggtct cacctgcgct ggggttagtc cgtgggcttg acagagaact
     1561 ggtagaaggc gttggacggt ttcaagagcc tgtttgccac cgtcatgact agcaatcgct
     1621 acaacttgct ccggagtcag accgtgggct tgacagagaa ctggcagtag gcgctgtacg
     1681 gtttcaagag cctgtttgcc accattgtta ctagcgatgg ccaccacctg ctccggggcg
     1741 agaccatatc tggtcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     1801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1981 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2461 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatcca cagaatcagg
     2521 ggataacgca ggaaagaaca tgaattaatt ctcatgtttg acagcttatc atcgattagc
     2581 tttaatgcgg tagtttatca cagttaaa
//
