LOCUS       sequence-360892-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     537..553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     537..553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     558..577
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        558..576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(751..1941)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1570..1589
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1989..1994,1995..2011,2012..2017))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2144..2602,1..130))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2224..2243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
       61 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      121 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgacgc
      181 gtaactcacg ttaagggatt ttggtcatgg gtggctcgac cagatatggt ctcgtggtag
      241 cgattgctag taacaatggt ggcaaacagg ctcttgaaac cgtacagcgc ctactgccag
      301 ttctctgtca agcccacggt ctgactccgg agcaagttgt agcgattgct agtcatgacg
      361 gtggcaaaca ggctcttgaa accgtccaac gccttctacc agttctctgt caagcccacg
      421 gactaacccc agcgcaagtt gtagtgagac cagctttggt ctagaggagg catgatgata
      481 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
      541 aaacagctat gaccatgtaa tacgactcac tataggggat attttatttt ctcccaatca
      601 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatcaagggt
      661 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
      721 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
      781 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
      841 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga
      901 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
      961 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     1021 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     1081 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     1141 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     1201 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     1261 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     1321 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc
     1381 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     1441 cgccgccgca aggaatggtg catgtaagga gatggcgccc aacagtcccc cggccacggg
     1501 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1561 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1621 gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc
     1681 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
     1741 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
     1801 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
     1861 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     1921 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     1981 actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct
     2041 ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc
     2101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2581 gaagaacagt atttggtatc tg
//
