LOCUS       sequence-360894-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     885..901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     885..901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     906..925
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        906..924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1099..2289)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1918..1937
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2337..2342,2343..2359,2360..2365))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2492..2603,1..477))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2572..2591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
       61 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      301 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgacgcgta actcacgtta
      541 agggattttg gtcatgggtg gctcgaccag atatggtctc ggtagcgatt gctagtaaca
      601 atggtggcaa acaggctctt gaaaccgtac agcgcctact gccagttctc tgtcaagccc
      661 acggtctgac tccggagcaa gttgtagcga ttgctagtca tgacggtggc aaacaggctc
      721 ttgaaaccgt ccaacgcctt ctaccagttc tctgtcaagc ccacggacta accccagcgc
      781 aagttgtagc aatgagacca gctttggtct agaggaggca tgatgatata tttttatctt
      841 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga
      901 ccatgtaata cgactcacta taggggatat tttattttct cccaatcagg cttgatcccc
      961 agtaagtcaa aaaatagctc gacatactgt tcttccccga tcaagggttg gtttgcgcat
     1021 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     1081 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     1141 ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct
     1201 cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca atgatcgaag ttaggctggt
     1261 aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     1321 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     1381 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat
     1441 gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc
     1501 ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct
     1561 ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag
     1621 ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg
     1681 gaaggagctg actgggttga aggctctcaa gggcatgggt cggcgctctc ccttatgcga
     1741 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
     1801 gaatggtgca tgtaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     1861 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     1921 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     1981 gcgtccggcg tagagaatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt
     2041 ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc
     2101 gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg
     2161 actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac
     2221 caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt
     2281 agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta
     2341 aagctaatcg atgataagct gtcaaacatg agaattaatt catgttcttt cctgcgttat
     2401 cccctgattc tgtggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2461 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2521 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2581 aggcgtttcc ccctggaagc tcc
//
