LOCUS       sequence-360908-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(38..626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(118..137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1031..1047
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1031..1047
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1052..1071
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1052..1070
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1245..2435)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2064..2083
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2483..2488,2489..2505,2506..2511))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
       61 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      661 ctcagtggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg ctcgaccaga
      721 tatggtctca tgacgcaatc gctacaactt gcgctggggt tagtccgtgg gcttgacaga
      781 gaactggtag aaggcgttgg acggtttcaa gagcctgttt gccaccgtca tgactagcaa
      841 tcgctacaac ttgctccgga gtcagaccgt gggcttgaca gagaactggc agtaggcgct
      901 gtacggtttc aagagcctgt ttgccaccat tgttagagcg agaccagctt tggtctagag
      961 gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
     1021 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatttta
     1081 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     1141 ccccgatcaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
     1201 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
     1261 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
     1321 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
     1381 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
     1441 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     1501 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     1561 acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt
     1621 ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca
     1681 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag
     1741 agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg
     1801 acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc
     1861 atgggtcggc gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     1921 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgta aggagatggc gcccaacagt
     1981 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     2041 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     2101 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga gaatccacag gacgggtgtg
     2161 gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg
     2221 cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca
     2281 tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc
     2341 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
     2401 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
     2461 tttaactgtg ataaactacc gcattaaagc taatcgatga taagctgtca aacatgagaa
     2521 ttaattcatg ttctttcctg cgttatcccc tgattctgtg gataacgcag gaaagaacat
     2581 gtgagcaaaa ggccagcaaa
//
