LOCUS       sequence-360909-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pG9
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(106..125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     917..933
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     917..933
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     938..957
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        938..956
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1131..2321)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1950..1969
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2369..2374,2375..2391,2392..2397))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
       61 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      661 acgcgtaact cacgttaagg gattttggtc atgggtggct cgaccagata tggtctcaag
      721 aggcaatcgc tacaacttgc tccggagtca gaccgtgggc ttgacagaga actggcagta
      781 ggcgctgtac ggtttcaaga gcctgtttgc caccattgtt ggaacgagac cagctttggt
      841 ctagaggagg catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
      901 cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat
      961 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     1021 gttcttcccc gatcaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     1081 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     1141 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     1201 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     1261 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     1321 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     1381 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     1441 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     1501 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     1561 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     1621 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     1681 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1741 aagggcatgg gtcggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     1801 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgtaagga gatggcgccc
     1861 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     1921 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     1981 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg
     2041 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     2101 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     2161 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     2221 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     2281 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     2341 tagcaattta actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca
     2401 tgagaattaa ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaacc
//
