LOCUS       sequence-360917-        6698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector containing a yeast integration cassette for RPL18B-driven 
            expression of PDR16-Link-Scarlet(rfp), a marker for the lipid 
            droplet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Bean BDM, Whiteway M, Martin VJJ
  TITLE     The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and 
            Overexpression CRISPR-Cas9 Toolkit.
  JOURNAL   G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154.
  PUBMED    35708612
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154."
COMMENT     Sequence Label: pBBK84
            pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-S...
FEATURES             Location/Qualifiers
     source          1..6698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1069..1657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1558..1577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgccaatgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgcccc ctatttatcg gcattgagat tgtaaaggaa
     1921 gtaaaacgca tttttgcaga tctgttctct tatgtatttt taatcgtcct tgtatggaag
     1981 tatcaaaggg gacgttcttc acctccttgg aaaacgcaaa ctttcttttt gcctatcaca
     2041 gtatcttatc gagctaacta ttttcgacac acatgaaaaa gcagaaatat taacgaaaaa
     2101 gaaaagaaag accatgtcat gtacgggcaa tcagaatatg caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaatcccac agtgagcaac
     2281 ttgacaaacc aacagatata ggctgtgtct taaaatatac catccaatac cttgatgaac
     2341 ttttcaaaga tgactaaaat aagtgaaatt tcaacattaa cttcgagctg taaacacagt
     2401 ggtttctttg cataaacacc atcagcctca agtcgtcaag taaagatttc gtgttcatgc
     2461 agatagataa caatctatat gttgataatt agcgttgcct catcaatgcg tacacgaaga
     2521 atatactaaa aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc
     2581 tagtaaagcg tattacaaat gaaaccaaga ttcagattgc gatccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatctatg ttcaagagat
     3541 ttagcaaaaa gaaggaggct ccagaagacc caaaaaattt gattaatatt gataagccta
     3601 tcaaagaact tcctgcttcg atagctattc caaaagaaaa accacttaca ggtgagcaac
     3661 aaaaaatgta tgacgaagtg ctaaaacatt tcagtaaccc tgacttgaag gtttatacaa
     3721 gtgaaaaaaa taagtcggag gatgatctta aacccttaga ggaagaagaa aaggcatggt
     3781 tgacaagaga atgtttttta cgttacctaa gggctactaa atgggtgcta aaagactgta
     3841 tcgatagaat caccatgact ctagcgtgga gaagagaatt cggtattagt catttaggtg
     3901 aagagcatgg tgataaaata actgctgact tggtggccgt cgagaacgaa tcaggtaagc
     3961 aggtgatttt gggttacgaa aacgatgcta gacctatctt atatttgaaa ccgggcagac
     4021 aaaatacaaa gacttctcac agacaggtac agcacttggt cttcatgttg gaaagagtta
     4081 tcgattttat gccagctggt caagactctc tagctttgct aatagacttt aaagattacc
     4141 ctgatgttcc taaagtacct ggcaatagta aaatcccacc tattggtgta gggaaagaag
     4201 tgctgcatat tctacaaact cattatccag aaagactagg aaaagcactt ttgacgaata
     4261 ttccttggtt agcatggact ttcttgaagt taattcaccc ttttattgac ccactgaccc
     4321 gtgaaaagct agtttttgat gaaccatttg ttaaatatgt ccctaagaac gaactagatt
     4381 cgttgtatgg tggtgatctg aaattcaagt acaatcatga tgtttattgg ccagctctgg
     4441 tggaaaccgc gcgtgaaaaa agagatcatt attttaagag gtttcaaagc ttcggtggta
     4501 ttgtaggttt aagtgaggtt gatttaagag gtactcatga aaaacttctt tacccagtaa
     4561 aatcggaaag cagtaccgtg ggatccggtc cttctggccc aactggcatg gttagtaaag
     4621 gtgaagctgt tataaaagaa ttcatgaggt ttaaagttca tatggaaggt tcaatgaatg
     4681 gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc atatgaaggt acacaaactg
     4741 ctaaattgaa agttactaaa ggtggtccat taccattttc ttgggatatt ttgtctccac
     4801 aattcatgta tggttctaga gcttttatta agcatccagc tgatattcca gattattata
     4861 aacaatcttt tcctgaaggt tttaaatggg aaagagttat gaatttcgaa gatggtggtg
     4921 ctgttactgt tactcaagat acttctttgg aagatggtac tttaatctat aaagttaaat
     4981 tgagaggtac taattttcca ccagatggtc cagttatgca aaagaaaact atgggttggg
     5041 aagcatctac tgaaagattg tatccagaag atggtgtttt gaaaggagac attaagatgg
     5101 ctttgagatt gaaagatggt ggtcggtacc tggccgactt caagaccacc tataaagcta
     5161 aaaaaccagt tcaaatgcca ggtgcatata atgttgatag aaagttagat ataacgtcgc
     5221 ataacgagga ctatactgta gttgaacaat atgaacgtag tgaaggtaga catagtaccg
     5281 gaggaatgga tgaattgtat aaatagctcg agtggcgcca attggtgcgg caattgataa
     5341 taacgaaaat gtcttttaat gatctgggta taatgaggaa ttttccgaac gtttttactt
     5401 tatatatata tatacatgta acatatattc tatacgctat agagaaagga aatttttcaa
     5461 ttaaaaaaaa aatagagaaa gagtttcact tcttgattat cgctaacact aatggttgaa
     5521 gtactgctac tttaatttta tgctgccaat gagacgacgg ggtcatcacg gctcatcatg
     5581 cgccaaacaa atgtgtgcaa tacacgctcg gatgactgca tgatgaccgc actgactggg
     5641 gacagcagat ccacctaagc ctgtgagaga agcagacacc cgacagatca aggcagttaa
     5701 ctagtgcact gcagtacaaa tcgtccccaa caaaagtggg ctctcaaaat tcatcacatt
     5761 taaatgcata taggaagagc aacagttggt ttgcatctga tgttccttaa agatttcgac
     5821 ataatgtgcg aagtagatcc cttcagcact gtacttgttt ttgcgactag attgtaaatc
     5881 attctttatt taatctcttt ctttaactac tgcttaaagt ataatttggt ccgtagttta
     5941 ataactatac taagcgaacg ctctctcgaa atttttctta acgcgtcctt gtactgcgtc
     6001 taacgctttt gccacttgga tttctattat aggaaatagt ctcacttact gggcgacgaa
     6061 ttttcgcgtt ttgatgaagc acaggaagaa tttctttatg ctcagaagtt tgacagcaag
     6121 caagttcatc attcgaacta gccttattgt tttagttcag tgacagcgaa ctgccgtact
     6181 cgatgcttta tttctcacgg tagagcggaa gaacagatag atccctacat attgctacca
     6241 cttctattac acaatagttt caatagcttg cagcgtagct aaactctaaa atttatctaa
     6301 atcactcata taaaccgaac ccttcccctt ccgcttatag tacagctgat gctccttcac
     6361 tattttaaca tgtggaattc ttgaaagaat gaaatcgcca tgccaagcca tcacacggtc
     6421 ttttatgcaa ttgattgacc gcctgcaaca cataggcagt aaaattttta caagcagtag
     6481 cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg tttctggacc
     6541 atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag tgccgagcgg
     6601 ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6661 tcaatctaaa gtatatatga gtaaacttgg tctgacag
//
