LOCUS       sequence-36095-e        7891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Uchida A, Alami NH, Brown A
  TITLE     Tight functional coupling of kinesin-1A and dynein motors in the 
            bidirectional transport of neurofilaments.
  JOURNAL   Mol Biol Cell. 2009 Dec . 20(23):4997-5006.
  PUBMED    19812246
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2009 Dec . 20(23):4997-5006."
COMMENT     Sequence Label: pKin1C
FEATURES             Location/Qualifiers
     source          1..7891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    4679..4800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4716..4735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4770..4789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4807..5262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4944..4965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5156..5175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5289..5393
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5395..5752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5395..5586
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5418..5438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5603..5738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5787..6581
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5787..6581
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5841..5860)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6451..6470
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6769..6788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6813..6860
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7189..7777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7678..7697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcggcacg aggcggtggt gctgatgcag
      661 gatggctgag cgcgaccgag cccgggaggt ctgagccggg cggggattgc tccctgagca
      721 tcgcctagtc cgcccgcctc gtggttgcgg gcaggtgggc cgggggcgca ggaccgggct
      781 ggaggtcagc agcagggcgg cccacctgat acgctgctct ccttcccggc ggcggagctg
      841 cggggcgtcc agcgcaggct tgagatgacc tgctgagaag ctgcgggagg cggcctagca
      901 gcgggcagcg gttggttcgc ggcctcctcg gtggcgctgc ccagccgggc cccccacccc
      961 atccctgtgc cccctcctgc tgcggcccga gatggcggat ccagccgaat gcagcatcaa
     1021 agtgatgtgc cggttccggc ccctcaacga agcggagatc ctccgcgggg acaaattcat
     1081 ccccaaattc aaaggcgagg agacggtggt gatcgggcaa gggaagccgt atgtctttga
     1141 ccgagtgctg ccgcccaaca caacccagga gcaggtctac aatgcctgtg caaagcagat
     1201 tgtcaaagat gtccttgagg gttataatgg aacaattttt gcatatgggc agacttcatc
     1261 aggaaaaact cataccatgg aggggaagtt acatgatcct cagcttatgg gtatcattcc
     1321 aaggattgca catgatattt ttgatcacat ctattccatg gacgagaacc tggagtttca
     1381 tatcaaggtt tcctattttg agatctactt ggacaaaata agggacttgc ttgatgtgtc
     1441 caagaccaac ttggcagttc atgaagacaa aaacagagtc ccctatgtaa aggggtgcac
     1501 cgagaggttt gtgtcaagcc cggaggaggt catggatgtg atcgatgagg gcaaagcaaa
     1561 ccgacacgtg gctgtgacaa acatgaacga acacagctct aggagtcaca gtatcttcct
     1621 gattaacatt aagcaagaga atgtggagac tgaaaaaaaa ctcagcggga agctgtattt
     1681 ggttgatttg gctgggagtg aaaaggtcag caaaaccggt gccgagggag ctgttcttga
     1741 tgaagctaaa aatatcaaca agtctttgtc tgctcttgga aatgtgattt ctgccttggc
     1801 agaagggaca aaaacacatg taccgtaccg ggacagcaag atgactcgga ttctccagga
     1861 ctctctgggt gggaactgta ggaccaccat tgtcatttgc tgttctcctt cagtcttcaa
     1921 tgaagccgag accaagtcca cgctgatgtt tggacagaga gcaaagacca tcaagaatac
     1981 agtctctgtg aacttggaac taacagcaga agagtggaag aagaaatatg aaaaagagaa
     2041 agagaagaac aaggccttga agagtgtcct ccagcatctg gagatggagc tgaacaggtg
     2101 gaggaacggg gaagctgtac ccgaggacga acagatcagc gccaaggacc agaagagcct
     2161 agagccctgt gacaacacac ccatcataga caacatcacg cctgttgtgg acggcatctc
     2221 tgccgagaag gagaagtatg acgaggagat caccagtttg taccgacagc tcgatgataa
     2281 ggatgatgaa attaaccagc agagccagct ggctgaaaag ctgaagcaac agatgttgga
     2341 tcaggatgag ctcctggctt ccacgagaag ggactatgag aagatccagg aggagctgac
     2401 acgcctccag atcgaaaatg aggcagctaa agacgaagtg aaagaagtcc tccaggccct
     2461 ggaggagctg gctgtcaatt acgaccagaa gtcacaagaa gtggaggaca agaccagggc
     2521 caacgagcaa ctgactgatg agctggccca gaaaacgacg acactgacaa ccacccagcg
     2581 agagctgagt cagctgcaag agcttagtaa ccaccagaaa aagagggcca cagagatcct
     2641 gaacctgctt ctcaaggacc tgggggagat aggcggaatt attggcacca acgatgtgaa
     2701 gactctggca gatgtgaacg gggtcattga ggaggagttc accatggcac gcctgtacat
     2761 tagcaagatg aagtcggagg tcaagtctct cgtgaaccgc agcaagcagc tggagagtgc
     2821 ccagatggac tctaacagga agangaacgc cagtgagcgc gagctggcag cgtgccagtt
     2881 gcttatctca cagcacgaag ccaagatcaa gtctctgaca gactacatgc agaacatgga
     2941 acagaagagg cggcagctgg aagagtccca ggactccctc agcgaggaac tggccaagct
     3001 ccgggcccag gaaaaaatgc acgaagtcag tttccaagat aaggaaaagg agcacctgac
     3061 gaggctgcag gatgctgagg aggtgaagaa agctctggag cagcagatgg agagccaccg
     3121 ggaagcgcac caaaagcagc tgtccagact tcgcgatgag attgaggaga agcagagaat
     3181 cattgatgag atccgggatt tgaatcagaa actgcaactg gaacaggaga ggctcagctc
     3241 tgattataac aagctgaaaa tagaggacca ggagagagaa gtgaaactgg agaagctcct
     3301 attgctcaat gacaaaaggg agcaagccag ggaggacctc aagggactgg aggagactgt
     3361 gtctagagaa ctccagaccc ttcataacct gcgcaaactc ttcgtccagg atttgacaac
     3421 ccgggtgaaa aagagtgtgg agctggacag cgacgatgga gggggcagcg ctgctcagaa
     3481 gcagaagatc tccttcctgg agaacaacct ggaacagctt accaaggtgc acaagcagct
     3541 ggtccgggac aatgcagatt tgcgctgtga actccccaag ctggagaaga ggcttcgtgc
     3601 taccgcagaa cgcgtcaagg ccttggagag tgcgctgaaa gaggccaagg agaatgccat
     3661 gagggaccga aaacgctacc agcaggaagt agatcgcatc aaggaggctg tgcgagccaa
     3721 gaacatggcc aggagggcac attcggctca gatcgccaag cccatccgcc caggccatta
     3781 cccagcatca tctccgacag ctgtccatgc cgtccgagga ggaggaggtg gctcttcaaa
     3841 ctctactcac taccagaaat aaagcagaat atgactccac gtagcatgaa aaggactgta
     3901 gtagtcacca gttcctttct gtcccctcct atacagtttc cgtttctttt tacacttgct
     3961 tgcccagccg tctgtagtac accagattca ggtgtttgcg ctgtgtagaa tttgtgtgca
     4021 cagatacgtg cttggatcct tctcctaaga agttggaagg agggggttac gcaataccca
     4081 cttcctgctc agacctcgcc acttctcagc ctccggggtt gtgtggtctc tggaggcccc
     4141 tgcagtgcac tggccccttg gaatggaacg ccctgctgtg ccatcctaga gggacaatca
     4201 agtgcagtgg aaccagtgac cacgctctgc ctcgacttct ggtttcctgt aaaataaaca
     4261 cattacttta tatttttagg gaacaaaaaa aaagtgctgc tatagggttc aaaattttca
     4321 ttctgaacac ttttccaaaa ccaaccccag agagcatttt aaatatcctg gtcatatctt
     4381 tggatctgta aaatagacct tttagtatgg cacctgttaa aatgcaaagc agattccttc
     4441 aaggcagaaa aacagtttga caatagcagt gtagaatttg ctcattcaga cacatctatg
     4501 aaaatgcgaa gagtcacaga attctgcagt cgacggtacc gcgggcccgg gatccaccgg
     4561 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4801 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4861 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4921 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4981 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     5041 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     5101 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     5161 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     5221 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     5281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     5401 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     5761 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5821 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5881 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5941 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     6001 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6061 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6121 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6181 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6241 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6301 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6361 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6421 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6481 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6541 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6601 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6661 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6721 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     6781 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     6841 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     6901 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     6961 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     7021 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7141 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7321 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7861 ttctgtggat aaccgtatta ccgccatgca t
//
