LOCUS       sequence-360963-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TT7
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(37..625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(117..136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1033..1049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1033..1049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1054..1073
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1054..1072
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1247..2437)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2066..2085
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2485..2490,2491..2507,2508..2513))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
       61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      661 tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc tcgaccagat
      721 atggtctcaa cttgcaatcg ctacaacttg ctcgggcgtc aggccgtggg cttgacagag
      781 aactggtaga aggcgctgaa cagtctcaag agcctgtttg ccacccccat tactagcaat
      841 cgctacaact tgctcgggcg tcaggccgtg ggcttgacag agaactggta gaaggcgctg
      901 aacagtctca agagcctgtt tgccaccccc attactggct cgagaccagc tttggtctag
      961 aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
     1021 ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata ggggatattt
     1081 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     1141 ttccccgatc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
     1201 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg
     1261 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta
     1321 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca
     1381 gcggtccaat gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct
     1441 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
     1501 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
     1561 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
     1621 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
     1681 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
     1741 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
     1801 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
     1861 gcatgggtcg gcgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     1921 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg taaggagatg gcgcccaaca
     1981 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     2041 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     2101 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gagaatccac aggacgggtg
     2161 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     2221 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     2281 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     2341 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     2401 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     2461 aatttaactg tgataaacta ccgcattaaa gctaatcgat gataagctgt caaacatgag
     2521 aattaattca tgttctttcc tgcgttatcc cctgattctg tggataacgc aggaaagaac
     2581 atgtgagcaa aaggccagca aaa
//
