LOCUS       sequence-360979-        4530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Tauzin A, Nicolas A, Ding S, Benlarbi M, Medjahed H, Chatterjee D, 
            Dionne K, Gong SY, Gendron-Lepage G, Bo Y, Perreault J, Goyette G, 
            Gokool L, Arlotto P, Morrisseau C, Tremblay C, Martel-Laferriere V, 
            De Serres G, Levade I, Kaufmann DE, Cote M, Bazin R, Finzi A
  TITLE     Spike recognition and neutralization of SARS-CoV-2 Omicron 
            subvariants elicited after the third dose of mRNA vaccine.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111998. doi: 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9.
  PUBMED    36656710
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111998. doi"; pages: " 
            10.1016/j.celrep.2023.111998. Epub 2023 Jan 9"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 BA.4/5 Spike
FEATURES             Location/Qualifiers
     source          1..4530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3876..3895)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3941..3996
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3995..4014)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4357..4373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4357..4373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4370..4392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4381..4397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4405..4411,4412..4429,4430..4435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4450..4471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca
       61 tgttcgtctt cctcgtcctc ctccccctcg tctcctccca gtgcgtcaac ctcatcaccc
      121 gcacccagag ctacaccaac tccttcaccc gcggcgtcta ctaccccgac aaggtcttcc
      181 gctcctccgt cctccactcc acccaggacc tcttcctccc cttcttctcc aacgtcacct
      241 ggttccacgc catctccggc accaacggca ccaagcgctt cgacaacccc gtcctcccct
      301 tcaacgacgg cgtctacttc gcctccaccg agaagtccaa catcatccgc ggctggatct
      361 tcggcaccac cctcgactcc aagacccagt ccctcctcat cgtcaacaac gccaccaacg
      421 tcgtcatcaa ggtctgcgag ttccagttct gcaacgaccc cttcctcgac gtctactacc
      481 acaagaacaa caagtcctgg atggagtccg agttccgcgt ctactcctcc gccaacaact
      541 gcaccttcga gtacgtctcc cagcccttcc tcatggacct cgagggcaag cagggcaact
      601 tcaagaacct ccgcgagttc gtcttcaaga acatcgacgg ctacttcaag atctactcca
      661 agcacacccc catcaacctc ggccgcgacc tcccccaggg cttctccgcc ctcgagcccc
      721 tcgtcgacct ccccatcggc atcaacatca cccgcttcca gaccctcctc gccctccacc
      781 gctcctacct cacccccggc gactcctcct ccggctggac cgccggcgcc gccgcctact
      841 acgtcggcta cctccagccc cgcaccttcc tcctcaagta caacgagaac ggcaccatca
      901 ccgacgccgt cgactgcgcc ctcgaccccc tctccgagac caagtgcacc ctcaagtcct
      961 tcaccgtcga gaagggcatc taccagacct ccaacttccg cgtccagccc accgagtcca
     1021 tcgtccgctt ccccaacatc accaacctct gccccttcga cgaggtcttc aacgccaccc
     1081 gcttcgcctc cgtctacgcc tggaaccgca agcgcatctc caactgcgtc gccgactact
     1141 ccgtcctcta caacttcgcc cctttcttcg ccttcaagtg ctacggcgtc tcccccacca
     1201 agctcaacga cctctgcttc accaacgtct acgccgactc cttcgtcatc cgcggcaacg
     1261 aggtcagcca gatcgccccc ggccagaccg gcaacatcgc cgactacaac tacaagctcc
     1321 ccgacgactt caccggctgc gtcatcgcct ggaactccaa caagctcgac tccaaggtcg
     1381 gcggcaacta caactacagg taccgcctct tccgcaagtc caacctcaag cccttcgagc
     1441 gcgacatctc caccgagatc taccaggccg gcaacaaacc ctgcaacggc gtcgccggcg
     1501 tgaactgcta cttccccctc cagtcctacg gcttcaggcc cacctacggc gtcggccacc
     1561 agccctaccg cgtcgtcgtc ctctccttcg agctcctcca cgcccccgcc accgtctgcg
     1621 gccccaagaa gtccaccaac ctcgtcaaga acaagtgcgt caacttcaac ttcaacggcc
     1681 tcaccggcac cggcgtcctc accgagtcca acaagaagtt cctccccttc cagcagttcg
     1741 gccgcgacat cgccgacacc accgacgccg tccgcgaccc ccagaccctc gagatcctcg
     1801 acatcacccc ctgctccttc ggcggcgtct ccgtcatcac ccccggcacc aacacctcca
     1861 accaggtcgc cgtcctctac cagggtgtca actgcaccga ggtccccgtc gccatccacg
     1921 ccgaccagct cacccccacc tggcgcgtct actccaccgg ctccaacgtc ttccagaccc
     1981 gcgccggctg cctcatcggc gccgagtacg tcaacaactc ctacgagtgc gacatcccca
     2041 tcggcgccgg catctgcgcc tcctaccaga cccagaccaa gtcccaccgc cgcgcccgct
     2101 ccgtcgcctc ccagtccatc atcgcctaca ccatgtccct cggcgccgag aactccgtcg
     2161 cctactccaa caactccatc gccatcccca ccaacttcac catctccgtc accaccgaga
     2221 tcctccccgt ctccatgacc aagacctccg tcgactgcac catgtacatc tgcggcgact
     2281 ccaccgagtg ctccaacctc ctcctccagt acggctcctt ctgcacccag ctcaagcgcg
     2341 ccctcaccgg catcgccgtc gagcaggaca agaacaccca ggaggtcttc gcccaggtca
     2401 agcagatcta caagaccccc cccatcaagt acttcggcgg cttcaacttc tcccagatcc
     2461 tccccgaccc ctccaagccc tccaagcgct ccttcatcga ggacctcctc ttcaacaagg
     2521 tcaccctcgc cgacgccggc ttcatcaagc agtacggcga ctgcctcggc gacatcgccg
     2581 cccgcgacct catctgcgcc cagaagttca acggcctcac cgtcctcccc cccctcctca
     2641 ccgacgagat gatcgcccag tacacctccg ccctcctcgc cggcaccatc acctccggct
     2701 ggaccttcgg cgccggcgcc gccctccaga tccccttcgc catgcagatg gcctaccgct
     2761 tcaacggcat cggcgtcacc cagaacgtcc tctacgagaa ccagaagctc atcgccaacc
     2821 agttcaactc cgccatcggc aagatccagg actccctctc ctccaccgcc tccgccctcg
     2881 gcaagctcca ggacgtcgtc aaccacaacg cccaggccct caacaccctc gtcaagcagc
     2941 tctcctccaa gttcggcgcc atctcctccg tcctcaacga catcctgtcc cgcctcgaca
     3001 aggtcgaggc cgaggtccag atcgaccgcc tcatcaccgg ccgcctccag tccctccaga
     3061 cctacgtcac ccagcagctc atccgcgccg ccgagatccg cgcctccgcc aacctcgccg
     3121 ccaccaagat gtccgagtgc gtcctcggcc agtccaagcg cgtcgacttc tgcggcaagg
     3181 gctaccacct catgtccttc ccccagtccg ccccccacgg cgtcgtcttc ctccacgtca
     3241 cctacgtccc cgcccaggag aagaacttca ccaccgcccc cgccatctgc cacgacggca
     3301 aggcccactt cccccgcgag ggcgtcttcg tctccaacgg cacccactgg ttcgtcaccc
     3361 agcgcaactt ctacgagccc cagatcatca ccaccgacaa caccttcgtc tccggcaact
     3421 gcgacgtcgt catcggcatc gtcaacaaca ccgtctacga ccccctccag cccgagctcg
     3481 actccttcaa ggaggagctc gacaagtact tcaagaacca cacctccccc gacgtcgacc
     3541 tcggcgacat ctccggcatc aacgcctccg tcgtcaacat ccagaaggag atcgaccgcc
     3601 tcaacgaggt cgccaagaac ctcaacgagt ccctcatcga cctccaggag ctcggcaagt
     3661 acgagcagta catcaagtgg ccctggtaca tctggctcgg cttcatcgcc ggcctcatcg
     3721 ccatcgtcat ggtcaccatc atgctctgct gcatgacctc ctgctgctcc tgcctcaagg
     3781 gctgctgctc ctgcggctcc tgctgcaagt tcgacgagga cgactccgag cccgtcctca
     3841 agggcgtcaa gctccactac acctaagcta gcagatcttt ttccctctgc caaaaattat
     3901 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     3961 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     4021 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg
     4081 ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct
     4141 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     4201 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     4261 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga
     4321 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4441 gggtgcctaa tgagtgagct aactcacatt aattgcgtgc gctcactgcc cgctttccag
     4501 tcgggaaacc tgtcgtgcca gcggatccgc
//
