LOCUS       sequence-360980-        4585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 BA.2.12.1 Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Gong SY, Ding S, Benlarbi M, Chen Y, Vezina D, Marchitto L, 
            Beaudoin-Bussieres G, Goyette G, Bourassa C, Bo Y, Medjahed H, 
            Levade I, Pazgier M, Cote M, Richard J, Prevost J, Finzi A
  TITLE     Temperature Influences the Interaction between SARS-CoV-2 Spike from
            Omicron Subvariants and Human ACE2.
  JOURNAL   Viruses. 2022 Sep 30;14(10):2178. doi: 10.3390/v14102178.
  PUBMED    36298733
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2022-09-30"; volume: "14(10):2178. doi"; pages: " 
            10.3390/v14102178"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 BA.2.12.1 Spike
FEATURES             Location/Qualifiers
     source          1..4585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3882..3901)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3947..4002
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4001..4020)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4363..4379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4363..4379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4376..4398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4387..4403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4411..4417,4418..4435,4436..4441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4456..4477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcctgggca cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgccacca
       61 tgttcgtctt cctcgtcctc ctccccctcg tctcctccca gtgcgtcaac ctcatcaccc
      121 gcacccagag ctacaccaac tccttcaccc gcggcgtcta ctaccccgac aaggtcttcc
      181 gctcctccgt cctccactcc acccaggacc tcttcctccc cttcttctcc aacgtcacct
      241 ggttccacgc catccacgtc tccggcacca acggcaccaa gcgcttcgac aaccccgtcc
      301 tccccttcaa cgacggcgtc tacttcgcct ccaccgagaa gtccaacatc atccgcggct
      361 ggatcttcgg caccaccctc gactccaaga cccagtccct cctcatcgtc aacaacgcca
      421 ccaacgtcgt catcaaggtc tgcgagttcc agttctgcaa cgaccccttc ctcgacgtct
      481 actaccacaa gaacaacaag tcctggatgg agtccgagtt ccgcgtctac tcctccgcca
      541 acaactgcac cttcgagtac gtctcccagc ccttcctcat ggacctcgag ggcaagcagg
      601 gcaacttcaa gaacctccgc gagttcgtct tcaagaacat cgacggctac ttcaagatct
      661 actccaagca cacccccatc aacctcggcc gcgacctccc ccagggcttc tccgccctcg
      721 agcccctcgt cgacctcccc atcggcatca acatcacccg cttccagacc ctcctcgccc
      781 tccaccgctc ctacctcacc cccggcgact cctcctccgg ctggaccgcc ggcgccgccg
      841 cctactacgt cggctacctc cagccccgca ccttcctcct caagtacaac gagaacggca
      901 ccatcaccga cgccgtcgac tgcgccctcg accccctctc cgagaccaag tgcaccctca
      961 agtccttcac cgtcgagaag ggcatctacc agacctccaa cttccgcgtc cagcccaccg
     1021 agtccatcgt ccgcttcccc aacatcacca acctctgccc cttcgacgag gtcttcaacg
     1081 ccacccgctt cgcctccgtc tacgcctgga accgcaagcg catctccaac tgcgtcgccg
     1141 actactccgt cctctacaac ttcgcccctt tcttcgcctt caagtgctac ggcgtctccc
     1201 ccaccaagct caacgacctc tgcttcacca acgtctacgc cgactccttc gtcatccgcg
     1261 gcaacgaggt cagccagatc gcccccggcc agaccggcaa catcgccgac tacaactaca
     1321 agctccccga cgacttcacc ggctgcgtca tcgcctggaa ctccaacaag ctcgactcca
     1381 aggtcggcgg caactacaac taccagtacc gcctcttccg caagtccaac ctcaagccct
     1441 tcgagcgcga catctccacc gagatctacc aggccggcaa caaaccctgc aacggcgtcg
     1501 ccggcttcaa ctgctacttc cccctcaggt cctacggctt caggcccacc tacggcgtcg
     1561 gccaccagcc ctaccgcgtc gtcgtcctct ccttcgagct cctccacgcc cccgccaccg
     1621 tctgcggccc caagaagtcc accaacctcg tcaagaacaa gtgcgtcaac ttcaacttca
     1681 acggcctcac cggcaccggc gtcctcaccg agtccaacaa gaagttcctc cccttccagc
     1741 agttcggccg cgacatcgcc gacaccaccg acgccgtccg cgacccccag accctcgaga
     1801 tcctcgacat caccccctgc tccttcggcg gcgtctccgt catcaccccc ggcaccaaca
     1861 cctccaacca ggtcgccgtc ctctaccagg gtgtcaactg caccgaggtc cccgtcgcca
     1921 tccacgccga ccagctcacc cccacctggc gcgtctactc caccggctcc aacgtcttcc
     1981 agacccgcgc cggctgcctc atcggcgccg agtacgtcaa caactcctac gagtgcgaca
     2041 tccccatcgg cgccggcatc tgcgcctcct accagaccca gaccaagtcc caccgccgcg
     2101 cccgctccgt cgcctcccag tccatcatcg cctacaccat gtccctcggc gccgagaacc
     2161 tggtcgccta ctccaacaac tccatcgcca tccccaccaa cttcaccatc tccgtcacca
     2221 ccgagatcct ccccgtctcc atgaccaaga cctccgtcga ctgcaccatg tacatctgcg
     2281 gcgactccac cgagtgctcc aacctcctcc tccagtacgg ctccttctgc acccagctca
     2341 agcgcgccct caccggcatc gccgtcgagc aggacaagaa cacccaggag gtcttcgccc
     2401 aggtcaagca gatctacaag acccccccca tcaagtactt cggcggcttc aacttctccc
     2461 agatcctccc cgacccctcc aagccctcca agcgctcctt catcgaggac ctcctcttca
     2521 acaaggtcac cctcgccgac gccggcttca tcaagcagta cggcgactgc ctcggcgaca
     2581 tcgccgcccg cgacctcatc tgcgcccaga agttcaacgg cctcaccgtc ctcccccccc
     2641 tcctcaccga cgagatgatc gcccagtaca cctccgccct cctcgccggc accatcacct
     2701 ccggctggac cttcggcgcc ggcgccgccc tccagatccc cttcgccatg cagatggcct
     2761 accgcttcaa cggcatcggc gtcacccaga acgtcctcta cgagaaccag aagctcatcg
     2821 ccaaccagtt caactccgcc atcggcaaga tccaggactc cctctcctcc accgcctccg
     2881 ccctcggcaa gctccaggac gtcgtcaacc acaacgccca ggccctcaac accctcgtca
     2941 agcagctctc ctccaagttc ggcgccatct cctccgtcct caacgacatc ctgtcccgcc
     3001 tcgacaaggt cgaggccgag gtccagatcg accgcctcat caccggccgc ctccagtccc
     3061 tccagaccta cgtcacccag cagctcatcc gcgccgccga gatccgcgcc tccgccaacc
     3121 tcgccgccac caagatgtcc gagtgcgtcc tcggccagtc caagcgcgtc gacttctgcg
     3181 gcaagggcta ccacctcatg tccttccccc agtccgcccc ccacggcgtc gtcttcctcc
     3241 acgtcaccta cgtccccgcc caggagaaga acttcaccac cgcccccgcc atctgccacg
     3301 acggcaaggc ccacttcccc cgcgagggcg tcttcgtctc caacggcacc cactggttcg
     3361 tcacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc ttcgtctccg
     3421 gcaactgcga cgtcgtcatc ggcatcgtca acaacaccgt ctacgacccc ctccagcccg
     3481 agctcgactc cttcaaggag gagctcgaca agtacttcaa gaaccacacc tcccccgacg
     3541 tcgacctcgg cgacatctcc ggcatcaacg cctccgtcgt caacatccag aaggagatcg
     3601 accgcctcaa cgaggtcgcc aagaacctca acgagtccct catcgacctc caggagctcg
     3661 gcaagtacga gcagtacatc aagtggccct ggtacatctg gctcggcttc atcgccggcc
     3721 tcatcgccat cgtcatggtc accatcatgc tctgctgcat gacctcctgc tgctcctgcc
     3781 tcaagggctg ctgctcctgc ggctcctgct gcaagttcga cgaggacgac tccgagcccg
     3841 tcctcaaggg cgtcaagctc cactacacct aagctagcag atctttttcc ctctgccaaa
     3901 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3961 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     4021 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     4081 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     4141 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     4201 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     4261 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4321 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     4381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4501 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaccatag
     4561 tcccgcccct aactccgccc atccc
//
