LOCUS       sequence-361-exp         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Shen S, Arhin GK, Ullu E, Tschudi C
  TITLE     In vivo epitope tagging of Trypanosoma brucei genes using a one step
            PCR-based strategy.
  JOURNAL   Mol Biochem Parasitol 2001 Mar;113(1):171-3.
  PUBMED    11254965
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol"; date: "2001-03"; volume: "113(1)"; pages: "171-3"
COMMENT     Sequence Label: BLA/Xpress
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..399
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             join(968..976,977..991)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
       61 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
      121 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
      181 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
      241 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
      301 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
      361 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg ccgaggagca
      421 ggactgacac gtgntaatta acgtgcggga ctctggggtt cgaaatgacc gaccaagcga
      481 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
      541 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
      601 agttcttcgc ccaccccaat tcgagctcgg tacccctaga aagtgtgaca acgtcgcacc
      661 atgtgtaggt tttcatttat gttctttctt tctttctttt gtgaatttgt tttctgtctc
      721 aaatgttttt aattcgcttg ggacctatgt ttttcttgtt tttttgctca ccctttgtgt
      781 aggaggcacc ctgtcacgtc tgtggttgcg tgtatgcctt ccttcccctt attcgcttct
      841 tcctgtcgtg tcacacctct ttctccctct ccctttccgc cttttctttc aatcttgttt
      901 tctcgaccag ccctactaga ggagaaagaa tagtaaccct ttcatcaaat agtccaaacg
      961 aattatggat ctgtacgacg atgacgataa g
//
