LOCUS       sequence-361121-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-hT8
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(209..214,215..231,232..237))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(364..952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(444..463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1225..1241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1225..1241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1246..1265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1246..1264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1439..2468,1..161))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2258..2277
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
       61 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      121 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      181 tagcaattta actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca
      241 tgagaattaa ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa
      301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      961 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga
     1021 ttttggtcat gggtggctcg accagatatg gtctcgaagt aatgggggcg gcaggccggc
     1081 gctggagagc attgttgccc agttatctcg ccctgatccg gcgttggccg cgtgagacca
     1141 gctttggtct agaggaggca tgatgatata tttttatctt gtgcaatgta acatcagaga
     1201 ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta
     1261 taggggatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     1321 gacatactgt tcttccccga tcaagggttg gtttgcgcat tcacagttct ccgcaagaat
     1381 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc
     1441 aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata
     1501 gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg
     1561 ccgtgacgat cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt
     1621 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     1681 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     1741 cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct
     1801 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     1861 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     1921 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     1981 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     2041 aggctctcaa gggcatgggt cggcgctctc ccttatgcga ctcctgcatt aggaagcagc
     2101 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgtaaggaga
     2161 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     2221 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     2281 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagagaatcc
     2341 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
     2401 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
     2461 attgcatc
//
