LOCUS       sequence-36118-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Venugopal A, Bryk R, Shi S, Rhee K, Rath P, Schnappinger D, Ehrt S, 
            Nathan C
  TITLE     Virulence of Mycobacterium tuberculosis depends on lipoamide 
            dehydrogenase, a member of three multienzyme complexes.
  JOURNAL   Cell Host Microbe. 2011 Jan 20. 9(1):21-31.
  PUBMED    21238944
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Host 
            Microbe. 2011 Jan 20. 9(1):21-31."
COMMENT     Sequence Label: pGMCZq17-OXOX
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     133..152
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        133..151
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(339..357)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(340..357)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    420..440
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    446..470
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(590..614)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(618..638)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(658..674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(658..674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(671..693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcggccagtg
      121 aaacgcgtaa attaatacga ctcactatag ggagaccaca acggtttcca tatggcatgc
      181 ttgaccgttg cgcaggaatt ccgactcgaa ctagggtggt gcggatggag gagcggcgtg
      241 ttggacttct taagcttgaa caccagatgg acacgctcca ggactactac atggttgcgc
      301 ggcacgtacc gatgccagct aggccatcga attccacttc tatagtgtca cctaaatcac
      361 cggttataaa aaatttttaa aataaaaaag gggacctcta gggtcccctg cagcttaagc
      421 aactatgtat aataaagttg acgcgtccac tttgtacaag aaagctgggt tcgcgcctac
      481 ctgctcaaag agagcctgcg gcatgtgttt tcggtcaagg gcgaggaagg taaacaggcc
      541 ctggaccggt ggatctcctg ggcccagcgc tgtcgcatcc cggtattcga gcctgctttt
      601 ttgtacaaac ttgaattcaa cttttctata caaagttgat agcttggcgt aatcatggtc
      661 atagctgttt cctgtgtgaa attgttatcc gctggtatca gctcactcaa aggcggtaat
      721 acggttatcc acagaatcag gggataacgc aggaaagaac atggtgacct catgtgctag
      781 agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga ccgctgcaac
      841 tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa tctggtgtga
      901 atgcccctcg tctgttcgcg caggcggggg g
//
