LOCUS       sequence-361193-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(351..356,357..373,374..379))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(506..1094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(586..605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1499..1515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1499..1515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1520..1539
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1520..1538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1713..2600,1..303))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2532..2551
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcgtagaga atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
       61 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
      121 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
      181 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
      241 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
      301 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta
      361 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
      421 attctgtgga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1141 acgcgtaact cacgttaagg gattttggtc atgggtggct cgaccagata tggtctcatg
     1201 acgcaatcgc tacaacttgc tccggagtca gaccgtgggc ttgacagaga actggcagta
     1261 ggcgctgtac ggtttcaaga gcctgtttgc caccattgtt actagcaatc gctacaactt
     1321 gctcgggcgt caggccgtgg gcttgacaga gaactggtag aaggcgctga acagtctcaa
     1381 gagcctgttt gccaccccca ttagagcgag accagctttg gtctagagga ggcatgatga
     1441 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca
     1501 ggaaacagct atgaccatgt aatacgactc actatagggg atattttatt ttctcccaat
     1561 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatcaagg
     1621 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
     1681 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
     1741 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     1801 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc
     1861 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     1921 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     1981 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     2041 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
     2101 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     2161 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     2221 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     2281 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat gggtcggcgc
     2341 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     2401 accgccgccg caaggaatgg tgcatgtaag gagatggcgc ccaacagtcc cccggccacg
     2461 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     2521 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     2581 atgccggcca cgatgcgtcc
//
