LOCUS       sequence-361199-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     378..394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     378..394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     399..418
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        399..417
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(592..1782)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1411..1430
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1830..1835,1836..1852,1853..1858))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1985..2573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2065..2084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgacgctc agtggaacga cgcgtaactc acgttaaggg attttggtca tgggtggctc
       61 gaccagatat ggtctcagga agcaatcgct acaacttgct ccggagtcag accgtgggct
      121 tgacagagaa ctggcagtag gcgctgtacg gtttcaagag cctgtttgcc accattgtta
      181 ctagcaatcg ctacaacttg ctcgggcgtc aggccgtggg cttgacagag aactggtaga
      241 aggcgctgaa cagtctcaag agcctgtttg ccacccccat tacttcgaga ccagctttgg
      301 tctagaggag gcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      361 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
      421 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
      481 tgttcttccc cgatcaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
      541 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
      601 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
      661 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
      721 gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
      781 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
      841 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
      901 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc
      961 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
     1021 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
     1081 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     1141 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     1201 caagggcatg ggtcggcgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1261 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgtaagg agatggcgcc
     1321 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1381 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1441 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagaa tccacaggac
     1501 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac
     1561 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat
     1621 caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat
     1681 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
     1741 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
     1801 ttagcaattt aactgtgata aactaccgca ttaaagctaa tcgatgataa gctgtcaaac
     1861 atgagaatta attcatgttc tttcctgcgt tatcccctga ttctgtggat aacgcaggaa
     1921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2401 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2581 ctttgatctt ttctacgggg
//
