LOCUS       sequence-36120-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Lokuta MA, Senetar MA, Bennin DA, Nuzzi PA, Chan KT, Ott VL, 
            Huttenlocher A
  TITLE     Type Igamma PIP kinase is a novel uropod component that regulates 
            rear retraction during neutrophil chemotaxis.
  JOURNAL   Mol Biol Cell. 2007 Dec . 18(12):5069-80.
  PUBMED    17928408
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2007 Dec . 18(12):5069-80."
COMMENT     Sequence Label: pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(81..100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(82..100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(145..348)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(174..194)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        349..728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg atccgagctc ggtaccaagc ttaagtttaa
       61 acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      121 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      181 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
      241 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
      301 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
      361 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
      421 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
      481 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
      541 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
      601 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
      661 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
      721 tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct
      781 aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct
      841 cgcgcactac tcagcgacct c
//
