LOCUS       sequence-361226-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at SF1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjSF1-tdTomato-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(116..121,122..139,140..146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    154..170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     159..181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     178..194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     178..194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             806..862
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             863..2293
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    2415..2536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2452..2471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2506..2525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2576..2609
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2652..3151
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2687..2710)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3084..3103
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3165..3968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    4296..4329
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5299..5871
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          5948..6013
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(6161..6180)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6226..6281
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6280..6299)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6626..6645)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6627..6645)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6652..6669)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6652..6668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6661..6683)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6809..7237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6896..6915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7106..7127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             7581..8375
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(8393..8461,8462..9253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8611..8630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9424..10012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9913..9932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10166..10183
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
       61 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      121 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      181 gaaacagcta tgaccatgat tacgccaagc ttcatgattc tcgttcattg tgaccacggt
      241 ggtgatgatg gcagtgatga tggtgacagg tgactgtgga tgggttgttg atggctggca
      301 ctggccacat ttatgaagag cacaggcacg tgggtgtcag tgacgatggt ggtgtttgtg
      361 aaccagtatg acaacaatgg tcatggtgac acggtcccag cagggagacc ctggttgcat
      421 ggttgatggt ggcagtggca agtcttcggt ggtcataggg aaggtggcat tgtgatgccg
      481 gtgacagtgt tggctgccca gccccaaagg ggatggatgg tgacccgatg gtgactgagc
      541 gcctcccttt gcagacgtga agttcctgaa taaccacagc ctggttaaag acgctcagga
      601 gaaggcgaac gctgccctgc tggactacac cctatgccac tacccacact gcggggacaa
      661 gtttcagcag ctgctgctgt gcctagtgga ggtgcgggcc ctgagcatgc aggccaagga
      721 gtacctgtac cacaagcacc tgggcaacga gatgccccgc aacaacctgc tcatcgagat
      781 gctgcaagct aagcagactg ctagcgccac taacttctcc ctgttgaaac aagcagggga
      841 tgtcgaagag aatcccgggc caatggtgag caagggcgag gaggtcatca aagagttcat
      901 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
      961 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     1021 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     1081 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     1141 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     1201 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     1261 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     1321 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     1381 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     1441 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1501 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     1561 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     1621 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     1681 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1741 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     1801 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     1861 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     1921 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     1981 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     2041 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     2101 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     2161 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     2221 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     2281 gctgtacaag taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     2341 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2401 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2521 tcatcaatgt atcttaaggc gttaggaatt ccgatcatat tcaataaccc ttaatataac
     2581 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
     2641 gctaatctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2701 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2761 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2821 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2881 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2941 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     3001 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     3061 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3121 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
     3181 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3241 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3301 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     3361 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3421 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3481 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3541 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3601 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3661 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3721 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3781 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     3841 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     3901 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3961 tcttctgagg ggatccgctg taagtctgca gaaattgatg atctattaaa caataaagat
     4021 gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag aacagagtac
     4081 ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg attagataaa
     4141 tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc atagttggat
     4201 atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc actcatgatc
     4261 tatagatccc tcgatcgaga tccggaaccc ttaatataac ttcgtataat gtatgctata
     4321 cgaagttatt aggtccctcg tgagcctggg cagggggcgg ggccggggct gggggcgggg
     4381 ccagggcggg gctgccacca ccccaccggc tccacgtgtt tcattttccg aggcccaccc
     4441 aggagccccg gccccgtccc cgtcccagag gccgctgccc ctgagttctg acacggtgtg
     4501 tttgggaagg tgggtgaggc tgggcagggc ctggcagagg tggggtggcc ctggccagcc
     4561 accagcactt gcctgctagt tggagtgccc aaaggaggtg gctggtaacc acccccgcca
     4621 gcccctgccc tgctcccaga tcttcagaat cgctcctgga ttctgaagcc tgcaggtcgg
     4681 gggaggaggt tcgggattcc ctagtgggcc tcgatgtccc ttggatcaga ggtcatccct
     4741 tcctcctctc ctgaaaacag acagggagaa gttgagcagg tatcaactgg gggagggaga
     4801 gggtctccag tgttccccta tggagaacag gagggaggtc ctctgttttg taaactaagg
     4861 ataactgagt ttgctaaact gggaggggct actgggccct agaggacagc aggagaactc
     4921 gttaggacaa agagtccccc tccctagccc ttctgctcca cctgactctg caagagggag
     4981 ctttgatcat catcaggaaa gaactttgct gcagtgtggt tttcaagagg aagcaaaaag
     5041 cctctccacc caggcctgga atgtttccac ccaatgtcga gcagtgtggt tttgcaagag
     5101 gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg agcaaacccc
     5161 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     5221 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     5281 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac
     5341 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa
     5401 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc
     5461 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa
     5521 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg
     5581 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag
     5641 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc
     5701 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg
     5761 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat
     5821 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt
     5881 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     5941 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     6001 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga
     6061 tccactagag tcgaagcggc ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     6121 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     6181 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     6241 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     6301 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     6361 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     6421 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     6481 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     6541 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     6601 ggagatctct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
     6661 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6721 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6781 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     6841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
     7261 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     7321 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     7381 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     7441 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     7501 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     7561 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7621 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7681 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7741 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7801 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7861 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
     7921 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7981 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     8041 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     8101 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     8161 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8221 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8281 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8341 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
     8401 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8461 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8521 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8581 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8641 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8701 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8761 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8821 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8881 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8941 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9001 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9061 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9121 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9181 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9241 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10081 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10141 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10201 aatacgcaaa ccgcctctcc
//
