LOCUS       sequence-361237-        7854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Reint G, Li Z, Labun K, Keskitalo S, Soppa I, Mamia K, Tolo E, 
            Szymanska M, Meza-Zepeda LA, Lorenz S, Cieslar-Pobuda A, Hu X, 
            Bordin DL, Staerk J, Valen E, Schmierer B, Varjosalo M, Taipale J, 
            Haapaniemi E
  TITLE     Rapid genome editing by CRISPR-Cas9-POLD3 fusion.
  JOURNAL   Elife. 2021 Dec 13;10. pii: 75415. doi: 10.7554/eLife.75415.
  PUBMED    34898428
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-12-13"; volume: "10. pii: 75415. doi"; pages: " 
            10.7554/eLife.75415"
COMMENT     Sequence Label: pDONR221 Cas9-POLD3
FEATURES             Location/Qualifiers
     source          1..7854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             674..721
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4877..4897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(5979..6078)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6095..6114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6096..6114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6119..6135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6119..6135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6237..6256)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6248..7057
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6315..6334)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7204..7792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7693..7712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atgaaaaggc cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa
      721 ggacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      781 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      841 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      901 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      961 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     1021 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     1081 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     1141 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     1201 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     1261 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     1321 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     1381 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggcaacct
     1441 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     1501 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     1561 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     1621 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     1681 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     1741 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     1801 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     1861 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     1921 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     1981 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     2041 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     2101 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     2161 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     2221 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     2281 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     2341 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     2401 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     2461 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     2521 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     2581 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     2641 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     2701 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     2761 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     2821 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     2881 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     2941 gaaggtggtg gacgagctgg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     3001 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     3061 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     3121 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     3181 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccacat
     3241 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     3301 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     3361 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     3421 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     3481 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     3541 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     3601 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     3661 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     3721 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     3781 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     3841 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     3901 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     3961 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     4021 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     4081 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     4141 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     4201 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     4261 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     4321 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     4381 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     4441 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     4501 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     4561 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     4621 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     4681 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     4741 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     4801 cctgtctcag ctgggaggcg acagcgctgg aggaggtgga agcggaggag gaggaagcgg
     4861 aggaggaggt agcggaccta agaaaaagag gaaggtgatg ctaaaggaca gtgggcctct
     4921 gttcaatact gactatgaca tccttaaaag caacttgcag aactgcagca aatttagtgc
     4981 tatacaatgt gcagctgccg tccctagagc tcctgctgaa tcctcttcgt cttccaaaaa
     5041 gtttgagcag tcacatcttc acatgtcaag tgagacacaa gccaacaatg agctgaccac
     5101 caatggtcat ggcccacctg catccaagca ggtttcccag cagcccaaag gaattatggg
     5161 aatgtttgcc tccaaagctg ctgctaaaac ccaagaaacc aacaaggaaa cgaaaacaga
     5221 ggctaaagaa gtaacaaatg catctgcagc aggcaacaag gcaccaggga aagggaatat
     5281 gatgagcaac ttttttggaa aagctgctat gaataaattt aaagtcaatt tggactcaga
     5341 acaagcagtg aaagaagaaa aaatagtgga gcagcctaca gtgtctgtca cggaaccaaa
     5401 gctggcaact cctgcaggcc tgaaaaaatc cagcaaaaaa gcagagcctg ttaaggtgct
     5461 gcagaaggaa aaaaaaaggg ggaagcgagt agcattatct gatgatgaga caaaggaaac
     5521 tgaaaacatg aggaaaaaga ggagaagaat caaacttcct gaatctgata gcagtgaaga
     5581 tgaagtcttt ccagactctc ctggggctta tgaagctgag tcaccatccc cacctcctcc
     5641 tccgtctcca cctcttgaac cagtgccaaa gactgagcct gaacctcctt ctgtcaagag
     5701 ctcaagtgga gaaaacaaaa gaaaacgaaa acgcgtacta aaatctaaaa cttacctgga
     5761 tggggaaggc tgcatagtga ctgaaaaagt ctacgagagt gaatcctgca cagatagtga
     5821 agaggagctt aacatgaaga catcctcagt acacagaccc cctgccatga ctgtgaaaaa
     5881 agaacccaga gaggaacgaa agggccccaa gaaagggact gctgctctgg gcaaagccaa
     5941 cagacaggtg tccattactg gcttcttcca gaggaaagac ccagctttct tgtacaaagt
     6001 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     6061 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     6121 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     6181 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     6241 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     6301 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     6361 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     6421 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     6481 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     6541 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     6601 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     6661 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     6721 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     6781 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     6841 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     6901 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     6961 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     7021 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     7081 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     7141 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
