LOCUS       sequence-36124-e       15037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, 
            Whelan SP, Sabatini B, Cepko CL
  TITLE     Anterograde or retrograde transsynaptic labeling of CNS neurons with
            vesicular stomatitis virus vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9. Epub 2011 Aug
            8.
  PUBMED    21825165
REFERENCE   2  (bases 1 to 15037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-09-13"; volume: "108(37)"; pages: 
            "15414-9. Epub 2011 Aug 8"
COMMENT     Sequence Label: pVSV eGFP RABV-G
FEATURES             Location/Qualifiers
     source          1..15037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(73..75,76..78,79..792)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(73..75,76..78,79..789)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(379..398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     726..747
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(12105..12123)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12119..12166
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(12234..12252)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12235..12252)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12542..12561)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(12771..12790)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12890..12912
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12950..12968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13036..13140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13141..13209,13210..14001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13359..13378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14172..14760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14661..14680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14914..14931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        15019..15037
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgaagacaa acaaaccatt attatcatta aaaggctcag gagaaacttt aacagtaatc
       61 agaattctcg agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      541 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      781 ctgtacaagt gatggccata tgaaaaaaac taacagtaat caaaatgtct gttacagtca
      841 agagaatcat tgacaacaca gtcatagttc caaaacttcc tgcaaatgag gatccagtgg
      901 aatacccggc agattacttc agaaaatcaa aggagattcc tctttacatc aatactacaa
      961 aaagtttgtc agatctaaga ggatatgtct accaaggcct caaatccgga aatgtatcaa
     1021 tcatacatgt caacagctac ttgtatggag cattaaagga catccggggt aagttggata
     1081 aagattggtc aagtttcgga ataaacatcg ggaaagcagg ggatacaatc ggaatatttg
     1141 accttgtatc cttgaaagcc ctggacggcg tacttccaga tggagtatcg gatgcttcca
     1201 gaaccagcgc agatgacaaa tggttgcctt tgtatctact tggcttatac agagtgggca
     1261 gaacacaaat gcctgaatac agaaaaaagc tcatggatgg gctgacaaat caatgcaaaa
     1321 tgatcaatga acagtttgaa cctcttgtgc cagaaggtcg tgacattttt gatgtgtggg
     1381 gaaatgacag taattacaca aaaattgtcg ctgcagtgga catgttcttc cacatgttca
     1441 aaaaacatga atgtgcctcg ttcagatacg gaactattgt ttccagattc aaagattgtg
     1501 ctgcattggc aacatttgga cacctctgca aaataaccgg aatgtctaca gaagatgtaa
     1561 cgacctggat cttgaaccga gaagttgcag atgaaatggt ccaaatgatg cttccaggcc
     1621 aagaaattga caaggccgat tcatacatgc cttatttgat cgactttgga ttgtcttcta
     1681 agtctccata ttcttccgtc aaaaaccctg ccttccactt ctgggggcaa ttgacagctc
     1741 ttctgctcag atccaccaga gcaaggaatg cccgacagcc tgatgacatt gagtatacat
     1801 ctcttactac agcaggtttg ttgtacgctt atgcagtagg atcctctgcc gacttggcac
     1861 aacagttttg tgttggagat aacaaataca ctccagatga tagtaccgga ggattgacga
     1921 ctaatgcacc gccacaaggc agagatgtgg tcgaatggct cggatggttt gaagatcaaa
     1981 acagaaaacc gactcctgat atgatgcagt atgcgaaaag agcagtcatg tcactgcaag
     2041 gcctaagaga gaagacaatt ggcaagtatg ctaagtcaga atttgacaaa tgaccctata
     2101 attctcagat cacctattat atattatgct acatatgaaa aaaactaaca gatatcatgg
     2161 ataatctcac aaaagttcgt gagtatctca agtcctactc tcgtctagat caggcggtag
     2221 gagagataga tgagatcgaa gcacaacgag ctgaaaagtc caattatgag ttgttccaag
     2281 aggacggagt ggaagagcat actaggccct cttattttca ggcagcagat gattctgaca
     2341 cagaatctga accagaaatt gaagacaatc aaggcttgta tgtaccagat ccggaagctg
     2401 agcaagttga aggctttata caggggcctt tagatgacta tgcagatgag gacgtggatg
     2461 ttgtattcac ttcggactgg aaacagcctg agcttgaatc cgacgagcat ggaaagacct
     2521 tacggttgac attgccagag ggtttaagtg gagagcagaa atcccagtgg cttttgacga
     2581 ttaaagcagt cgttcaaagt gccaaacact ggaatctggc agagtgcaca tttgaagcat
     2641 cgggagaagg ggtcatcata aaaaagcgcc agataactcc ggatgtatat aaggtcactc
     2701 cagtgatgaa cacacatccg taccaatcag aagccgtatc agatgtttgg tctctctcaa
     2761 agacatccat gactttccaa cccaagaaag caagtcttca gcctctcacc atatccttgg
     2821 atgaattgtt ctcatctaga ggagaattca tctctgtcgg aggtaacgga cgaatgtctc
     2881 ataaagaggc catcctgctc ggtctgaggt acaaaaagtt gtacaatcag gcgagagtca
     2941 aatattctct gtagactatg aaaaaaagta acagatatca caatctaagt gttatcccaa
     3001 tccattcatc atgagttcct taaagaagat tctcggtctg aaggggaaag gtaagaaatc
     3061 taagaaatta gggatcgcac caccccctta tgaagaggac actagcatgg agtatgctcc
     3121 gagcgctcca attgacaaat cctattttgg agttgacgag atggacacct atgatccgaa
     3181 tcaattaaga tatgagaaat tcttctttac agtgaaaatg acggttagat ctaatcgtcc
     3241 gttcagaaca tactcagatg tggcagccgc tgtatcccat tgggatcaca tgtacatcgg
     3301 aatggcaggg aaacgtccct tctacaaaat cttggctttt ttgggttctt ctaatctaaa
     3361 ggccactcca gcggtattgg cagatcaagg tcaaccagag tatcacgctc actgcgaagg
     3421 cagggcttat ttgccacata ggatggggaa gacccctccc atgctcaatg taccagagca
     3481 cttcagaaga ccattcaata taggtcttta caagggaacg attgagctca caatgaccat
     3541 ctacgatgat gagtcactgg aagcagctcc tatgatctgg gatcatttca attcttccaa
     3601 attttctgat ttcagagaga aggccttaat gtttggcctg attgtcgaga aaaaggcatc
     3661 tggagcgtgg gtcctggatt ctatcagcca cttcaaatga gctagtctag cttccagctt
     3721 ctgaacaatc cccggtttac tcagtctctc ctaattccag cctttcgaac aactaatatc
     3781 ctgtcttttc tatccctatg aaaaaaacta acagagatcg atctgtttcc ttgacacgcg
     3841 tatggttcct caggctctcc tgtttgtacc ccttctggtt tttccattgt gttttgggaa
     3901 attccctatt tacacgatac cagacaagct tggtccctgg agtccgattg acatacatca
     3961 cctcagctgc ccaaacaatt tggtagtgga ggacgaagga tgcaccaacc tgtcagggtt
     4021 ctcctacatg gaacttaaag ttggatacat cttagccata aaagtgaacg ggttcacttg
     4081 cacaggcgtt gtgacggagg ctgaaaccta cactaacttc gttggttatg tcacaaccac
     4141 gttcaaaaga aagcatttcc gcccaacacc agatgcatgt agagccgcgt acaactggaa
     4201 gatggccggt gaccccagat atgaagagtc tctacacaat ccgtaccctg actaccgctg
     4261 gcttcgaact gtaaaaacca ccaaggagtc tctcgttatc atatctccaa gtgtggcaga
     4321 tttggaccca tatgacagat cccttcactc gagggtcttc cctagcggga agtgctcagg
     4381 agtagcggtg tcttctacct actgctccac taaccacgat tacaccattt ggatgcccga
     4441 gaatccgaga ctagggatgt cttgtgacat ttttaccaat agtagaggga agagagcatc
     4501 caaagggagt gagacttgcg gctttgtaga tgaaagaggc ctatataagt ctttaaaagg
     4561 agcatgcaaa ctcaagttat gtggagttct aggacttaga cttatggatg gaacatgggt
     4621 ctcgatgcaa acatcaaatg aaaccaaatg gtgccctccc gataagttgg tgaacctgca
     4681 cgactttcgc tcagacgaaa ttgagcacct tgttgtagag gagttggtca ggaagagaga
     4741 ggagtgtctg gatgcactag agtccatcat gacaaccaag tcagtgagtt tcagacgtct
     4801 cagtcattta agaaaacttg tccctgggtt tggaaaagca tataccatat tcaacaagac
     4861 cttgatggaa gccgatgctc actacaagtc agtcagaact tggaatgaga tcctcccttc
     4921 aaaagggtgt ttaagagttg gggggaggtg tcatcctcat gtgaacgggg tgtttttcaa
     4981 tggtataata ttaggacctg acggcaatgt cttaatccca gagatgcaat catccctcct
     5041 ccagcaacat atggagttgt tggaatcctc ggttatcccc cttgtgcacc ccctggcaga
     5101 cccgtctacc gttttcaagg acggtgacga ggctgaggat tttgttgaag ttcaccttcc
     5161 cgatgtgcac aatcaggtct caggagttga cttgggtctc ccgaactggg ggaagtatgt
     5221 attactgagt gcaggggccc tgactgcctt gatgttgata attttcctga tgacatgttg
     5281 tagaagagtc aatcgatcag aacctacgca acacaatctc agagggacag ggagggaggt
     5341 gtcagtcact ccccaaagcg ggaagatcat atcttcatgg gaatcacaca agagtggggg
     5401 tgagaccaga ctgtaagcgg ccgccctgca caacagattc ttcatgtttg aaccaaatca
     5461 acttgtgata tcatgctcaa agaggcctta attatatttt aatttttaat ttttatgaaa
     5521 aaaactaaca gcaatcatgg aagtccacga ttttgagacc gacgagttca atgatttcaa
     5581 tgaagatgac tatgccacaa gagaattcct gaatcccgat gagcgcatga cgtacttgaa
     5641 tcatgctgat tacaatttga attctcctct aattagtgat gatattgaca atttgatcag
     5701 gaaattcaat tctcttccga ttccctcgat gtgggatagt aagaactggg atggagttct
     5761 tgagatgtta acatcatgtc aagccaatcc catctcaaca tctcagatgc ataaatggat
     5821 gggaagttgg ttaatgtctg ataatcatga tgccagtcaa gggtatagtt ttttacatga
     5881 agtggacaaa gaggcagaaa taacatttga cgtggtggag accttcatcc gcggctgggg
     5941 caacaaacca attgaataca tcaaaaagga aagatggact gactcattca aaattctcgc
     6001 ttatttgtgt caaaagtttt tggacttaca caagttgaca ttaatcttaa atgctgtctc
     6061 tgaggtggaa ttgctcaact tggcgaggac tttcaaaggc aaagtcagaa gaagttctca
     6121 tggaacgaac atatgcagga ttagggttcc cagcttgggt cctactttta tttcagaagg
     6181 atgggcttac ttcaagaaac ttgatattct aatggaccga aactttctgt taatggtcaa
     6241 agatgtgatt atagggagga tgcaaacggt gctatccatg gtatgtagaa tagacaacct
     6301 gttctcagag caagacatct tctcccttct aaatatctac agaattggag ataaaattgt
     6361 ggagaggcag ggaaattttt cttatgactt gattaaaatg gtggaaccga tatgcaactt
     6421 gaagctgatg aaattagcaa gagaatcaag gcctttagtc ccacaattcc ctcattttga
     6481 aaatcatatc aagacttctg ttgatgaagg ggcaaaaatt gaccgaggta taagattcct
     6541 ccatgatcag ataatgagtg tgaaaacagt ggatctcaca ctggtgattt atggatcgtt
     6601 cagacattgg ggtcatcctt ttatagatta ttacactgga ctagaaaaat tacattccca
     6661 agtaaccatg aagaaagata ttgatgtgtc atatgcaaaa gcacttgcaa gtgatttagc
     6721 tcggattgtt ctatttcaac agttcaatga tcataaaaag tggttcgtga atggagactt
     6781 gctccctcat gatcatccct ttaaaagtca tgttaaagaa aatacatggc ccacagctgc
     6841 tcaagttcaa gattttggag ataaatggca tgaacttccg ctgattaaat gttttgaaat
     6901 acccgactta ctagacccat cgataatata ctctgacaaa agtcattcaa tgaataggtc
     6961 agaggtgttg aaacatgtcc gaatgaatcc gaacactcct atccctagta aaaaggtgtt
     7021 gcagactatg ttggacacaa aggctaccaa ttggaaagaa tttcttaaag agattgatga
     7081 gaagggctta gatgatgatg atctaattat tggtcttaaa ggaaaggaga gggaactgaa
     7141 gttggcaggt agatttttct ccctaatgtc ttggaaattg cgagaatact ttgtaattac
     7201 cgaatatttg ataaagactc atttcgtccc tatgtttaaa ggcctgacaa tggcggacga
     7261 tctaactgca gtcattaaaa agatgttaga ttcctcatcc ggccaaggat tgaagtcata
     7321 tgaggcaatt tgcatagcca atcacattga ttacgaaaaa tggaataacc accaaaggaa
     7381 gttatcaaac ggcccagtgt tccgagttat gggccagttc ttaggttatc catccttaat
     7441 cgagagaact catgaatttt ttgagaaaag tcttatatac tacaatggaa gaccagactt
     7501 gatgcgtgtt cacaacaaca cactgatcaa ttcaacctcc caacgagttt gttggcaagg
     7561 acaagagggt ggactggaag gtctacggca aaaaggatgg agtatcctca atctactggt
     7621 tattcaaaga gaggctaaaa tcagaaacac tgctgtcaaa gtcttggcac aaggtgataa
     7681 tcaagttatt tgcacacagt ataaaacgaa gaaatcgaga aacgttgtag aattacaggg
     7741 tgctctcaat caaatggttt ctaataatga gaaaattatg actgcaatca aaatagggac
     7801 agggaagtta ggacttttga taaatgacga tgagactatg caatctgcag attacttgaa
     7861 ttatggaaaa ataccgattt tccgtggagt gattagaggg ttagagacca agagatggtc
     7921 acgagtgact tgtgtcacca atgaccaaat acccacttgt gctaatataa tgagctcagt
     7981 ttccacaaat gctctcaccg tagctcattt tgctgagaac ccaatcaatg ccatgataca
     8041 gtacaattat tttgggacat ttgctagact cttgttgatg atgcatgatc ctgctcttcg
     8101 tcaatcattg tatgaagttc aagataagat accgggcttg cacagttcta ctttcaaata
     8161 cgccatgttg tatttggacc cttccattgg aggagtgtcg ggcatgtctt tgtccaggtt
     8221 tttgattaga gccttcccag atcccgtaac agaaagtctc tcattctgga gattcatcca
     8281 tgtacatgct cgaagtgagc atctgaagga gatgagtgca gtatttggaa accccgagat
     8341 agccaagttt cgaataactc acatagacaa gctagtagaa gatccaacct ctctgaacat
     8401 cgctatggga atgagtccag cgaacttgtt aaagactgag gttaaaaaat gcttaatcga
     8461 atcaagacaa accatcagga accaggtgat taaggatgca accatatatt tgtatcatga
     8521 agaggatcgg ctcagaagtt tcttatggtc aataaatcct ctgttcccta gatttttaag
     8581 tgaattcaaa tcaggcactt ttttgggagt cgcagacggg ctcatcagtc tatttcaaaa
     8641 ttctcgtact attcggaact cctttaagaa aaagtatcat agggaattgg atgatttgat
     8701 tgtgaggagt gaggtatcct ctttgacaca tttagggaaa cttcatttga gaaggggatc
     8761 atgtaaaatg tggacatgtt cagctactca tgctgacaca ttaagataca aatcctgggg
     8821 ccgtacagtt attgggacaa ctgtacccca tccattagaa atgttgggtc cacaacatcg
     8881 aaaagagact ccttgtgcac catgtaacac atcagggttc aattatgttt ctgtgcattg
     8941 tccagacggg atccatgacg tctttagttc acggggacca ttgcctgctt atctagggtc
     9001 taaaacatct gaatctacat ctattttgca gccttgggaa agggaaagca aagtcccact
     9061 gattaaaaga gctacacgtc ttagagatgc tatctcttgg tttgttgaac ccgactctaa
     9121 actagcaatg actatacttt ctaacatcca ctctttaaca ggcgaagaat ggaccaaaag
     9181 gcagcatggg ttcaaaagaa cagggtctgc ccttcatagg ttttcgacat ctcggatgag
     9241 ccatggtggg ttcgcatctc agagcactgc agcattgacc aggttgatgg caactacaga
     9301 caccatgagg gatctgggag atcagaattt cgacttttta ttccaagcaa cgttgctcta
     9361 tgctcaaatt accaccactg ttgcaagaga cggatggatc accagttgta cagatcatta
     9421 tcatattgcc tgtaagtcct gtttgagacc catagaagag atcaccctgg actcaagtat
     9481 ggactacacg cccccagatg tatcccatgt gctgaagaca tggaggaatg gggaaggttc
     9541 gtggggacaa gagataaaac agatctatcc tttagaaggg aattggaaga atttagcacc
     9601 tgctgagcaa tcctatcaag tcggcagatg tataggtttt ctatatggag acttggcgta
     9661 tagaaaatct actcatgccg aggacagttc tctatttcct ctatctatac aaggtcgtat
     9721 tagaggtcga ggtttcttaa aagggttgct agacggatta atgagagcaa gttgctgcca
     9781 agtaatacac cggagaagtc tggctcattt gaagaggccg gccaacgcag tgtacggagg
     9841 tttgatttac ttgattgata aattgagtgt atcacctcca ttcctttctc ttactagatc
     9901 aggacctatt agagacgaat tagaaacgat tccccacaag atcccaacct cctatccgac
     9961 aagcaaccgt gatatggggg tgattgtcag aaattacttc aaataccaat gccgtctaat
    10021 tgaaaaggga aaatacagat cacattattc acaattatgg ttattctcag atgtcttatc
    10081 catagacttc attggaccat tctctatttc caccaccctc ttgcaaatcc tatacaagcc
    10141 atttttatct gggaaagata agaatgagtt gagagagctg gcaaatcttt cttcattgct
    10201 aagatcagga gaggggtggg aagacataca tgtgaaattc ttcaccaagg acatattatt
    10261 gtgtccagag gaaatcagac atgcttgcaa gttcgggatt gctaaggata ataataaaga
    10321 catgagctat cccccttggg gaagggaatc cagagggaca attacaacaa tccctgttta
    10381 ttatacgacc accccttacc caaagatgct agagatgcct ccaagaatcc aaaatcccct
    10441 gctgtccgga atcaggttgg gccaattacc aactggcgct cattataaaa ttcggagtat
    10501 attacatgga atgggaatcc attacaggga cttcttgagt tgtggagacg gctccggagg
    10561 gatgactgct gcattactac gagaaaatgt gcatagcaga ggaatattca atagtctgtt
    10621 agaattatca gggtcagtca tgcgaggcgc ctctcctgag ccccccagtg ccctagaaac
    10681 tttaggagga gataaatcga gatgtgtaaa tggtgaaaca tgttgggaat atccatctga
    10741 cttatgtgac ccaaggactt gggactattt cctccgactc aaagcaggct tggggcttca
    10801 aattgattta attgtaatgg atatggaagt tcgggattct tctactagcc tgaaaattga
    10861 gacgaatgtt agaaattatg tgcaccggat tttggatgag caaggagttt taatctacaa
    10921 gacttatgga acatatattt gtgagagcga aaagaatgca gtaacaatcc ttggtcccat
    10981 gttcaagacg gtcgacttag ttcaaacaga atttagtagt tctcaaacgt ctgaagtata
    11041 tatggtatgt aaaggtttga agaaattaat cgatgaaccc aatcccgatt ggtcttccat
    11101 caatgaatcc tggaaaaacc tgtacgcatt ccagtcatca gaacaggaat ttgccagagc
    11161 aaagaaggtt agtacatact ttaccttgac aggtattccc tcccaattca ttcctgatcc
    11221 ttttgtaaac attgagacta tgctacaaat attcggagta cccacgggtg tgtctcatgc
    11281 ggctgcctta aaatcatctg atagacctgc agatttattg accattagcc ttttttatat
    11341 ggcgattata tcgtattata acatcaatca tatcagagta ggaccgatac ctccgaaccc
    11401 cccatcagat ggaattgcac aaaatgtggg gatcgctata actggtataa gcttttggct
    11461 gagtttgatg gagaaagaca ttccactata tcaacagtgt ttggcagtta tccagcaatc
    11521 atttccgatt aggtgggagg ctatttcagt aaaaggagga tacaagcaga agtggagtac
    11581 tagaggtgat gggctcccaa aagatacccg aatttcagac tccttggccc caatcgggaa
    11641 ctggatcaga tctttggaat tggtccgaaa ccaagttcgt ctaaatccat tcaataagat
    11701 cttgttcaat cagctatgtc gtacagtgga taatcatttg aagtggtcaa atttgcgaaa
    11761 aaacacagga atgattgaat ggatcaatgg gcgaatttca aaagaagacc ggtctatact
    11821 gatgttgaag agtgacctac atgaggaaaa ctcttggaga gattaaaaaa tcaggaggag
    11881 actccaaact ttaagtatga aaaaaacttt gatccttaag accctcttgt ggtttttatt
    11941 tttttatctg gttttgtggt cttcgtgggt cggcatggca tctccacctc ctcgcggtcc
    12001 gacctgggca tccgaaggag gacgtcgtcc actcggatgg ctaagggaga gctcggatcc
    12061 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
    12121 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
    12181 tatatccgga tcgagatcct ctagagtcga cctgcaggca tgcaagcttg tattctatag
    12241 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
    12301 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
    12361 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
    12421 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
    12481 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
    12541 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
    12601 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
    12661 tccttgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
    12721 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
    12781 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
    12841 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    12901 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
    12961 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
    13021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    13081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    13141 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    13201 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    13261 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    13321 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    13381 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    13441 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    13501 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    13561 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    13621 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    13681 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    13741 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    13801 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    13861 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    13921 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    13981 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    14041 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    14101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    14161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    14221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    14281 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    14341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    14401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    14461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    14521 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    14581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    14641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    14701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    14761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    14821 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    14881 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    14941 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcaggggg
    15001 atctcgatcc cgcgaaatta atacgactca ctatagg
//
