LOCUS       sequence-361260-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     618..637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        618..636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(811..2001)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1630..1649
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2049..2054,2055..2071,2072..2077))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2204..2603,1..189))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2284..2303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
       61 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg
      241 taactcacgt taagggattt tggtcatggg tggctcgacc agatatggtc tcggattgct
      301 agtaatgggg gtggcaaaca ggctcttgag actgttcagc gccttctacc agttctctgt
      361 caagcccacg gcctgacgcc cgagcaagtt gtagcgattg ctagtaacaa tggtggcaaa
      421 caggctcttg aaaccgtaca gcgcctactg ccagttctct gtcaagccca cggtctgact
      481 ccggagcaag ttgtagcgat agcctgagac cagctttggt ctagaggagg catgatgata
      541 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
      601 aaacagctat gaccatgtaa tacgactcac tataggggat attttatttt ctcccaatca
      661 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatcaagggt
      721 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
      781 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
      841 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
      901 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga
      961 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
     1021 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     1081 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     1141 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     1201 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     1261 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     1321 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     1381 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc
     1441 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     1501 cgccgccgca aggaatggtg catgtaagga gatggcgccc aacagtcccc cggccacggg
     1561 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1621 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1681 gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc
     1741 gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc
     1801 ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag
     1861 cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag
     1921 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
     1981 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
     2041 actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa ttcatgttct
     2101 ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga gcaaaaggcc
     2161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2581 cgaggtatgt aggcggtgct aca
//
