LOCUS       sequence-36127-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  TITLE     McCabe Lab Plasmids
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAd/CMV/PBX1-IRES-nEBFP2
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(289..309)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(313..333)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(337..357)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(546..565)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcac tgcacgccgt ggctcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg ttggtggcat cgccctcgcc
      181 ctcgcccctc acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctgagcatg atcacgtgta cctttctctt
      301 cttttttgga tctacctttc tcttcttttt tggatctacc tttctcttct tttttggatc
      361 cacgtgcacc atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc
      421 cgtggttcgg ggggcctaga cgttttttta acctccacta aacacatgta aagcatgtgt
      481 accgaggccc cagatcagat cccatacaat ggggtacctt ctgggcatcc ctcagcccct
      541 tgttgaatac gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt
      601 ggcactggg
//
