LOCUS       sequence-361285-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      382..970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     871..890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1097..1102,1103..1119,1120..1125)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1173..2363
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1525..1544)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2537..2556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2538..2556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2561..2577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2561..2577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccaaagctgg
       61 tctcaaccac ttgctccgga gtcagaccgt gggcttgaca gagaactggc agtaggcgct
      121 gtacggtttc aagagcctgt ttgccaccat tgttactagc aatcgctaca acttgctcgg
      181 gcgtcaggcc gtgggcttga cagagaactg gtagaaggcg ctgaacagtc tcaagagcct
      241 gtttgccacc cccattacta gcaatcgcta caacctgcga gaccatatct ggtcgagcca
      301 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
      361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1021 tgctcacatg ttctttcctg cgttatccac agaatcaggg gataacgcag gaaagaacat
     1081 gaattaattc tcatgtttga cagcttatca tcgattagct ttaatgcggt agtttatcac
     1141 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     1201 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     1261 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     1321 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     1381 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     1441 tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcc ggcatcaccg
     1501 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1561 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     1621 ccgggggact gttgggcgcc atctccttac atgcaccatt ccttgcggcg gcggtgctca
     1681 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgcc
     1741 gacccatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1801 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     1861 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     1921 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     1981 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     2041 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     2101 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     2161 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     2221 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     2281 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2341 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     2401 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     2461 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     2521 gagaaaataa aatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2581 gctctggccc gtgtctcaaa atct
//
