LOCUS       sequence-361287-        2608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     644..660
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     644..660
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     665..684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        665..683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(858..2048)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1677..1696
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2096..2101,2102..2118,2119..2124))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2251..2608,1..231))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2331..2350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
       61 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      121 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      181 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      241 ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat
      301 tttggtcatg ggtggctcga ccagatatgg tctcgccccg gagcaggtgg tggccatcgc
      361 tagtaatggg ggtggcaaac aggctcttga gactgttcag cgccttctac cagttctctg
      421 tcaagcccac ggcctgacgc ccgagcaagt tgtagcgatt gctagtaaca atggtggcaa
      481 acaggctctt gaaaccgtac agcgcctact gccagttctc tgtcaagccc acggtctgac
      541 tccggagcag gtgagaccag ctttggtcta gaggaggcat gatgatatat ttttatcttg
      601 tgcaatgtaa catcagagat tttgagacac gggccagagc tgccaggaaa cagctatgac
      661 catgtaatac gactcactat aggggatatt ttattttctc ccaatcaggc ttgatcccca
      721 gtaagtcaaa aaatagctcg acatactgtt cttccccgat caagggttgg tttgcgcatt
      781 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
      841 gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg
      901 gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc
      961 gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta
     1021 agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
     1081 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
     1141 gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg
     1201 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     1261 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     1321 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     1381 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     1441 aaggagctga ctgggttgaa ggctctcaag ggcatgggtc ggcgctctcc cttatgcgac
     1501 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     1561 aatggtgcat gtaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     1621 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     1681 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     1741 cgtccggcgt agagaatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
     1801 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
     1861 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga
     1921 ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc
     1981 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta
     2041 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa
     2101 agctaatcga tgataagctg tcaaacatga gaattaattc atgttctttc ctgcgttatc
     2161 ccctgattct gtggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2281 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2341 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2401 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2461 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2521 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2581 cgacttatcg ccactggcag cagccact
//
