LOCUS       sequence-361312-       12147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human 7xHA- PEX6 fused to Tomato, and PEX6 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Haimovich G, Dasgupta S, Ravi AG, Gerst JE
  TITLE     Complementation of a human disease phenotype in vitro by 
            intercellular mRNA transfer
  JOURNAL   bioRxiv 2024.11.06.622258
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.06.622258"
COMMENT     Sequence Label: pHAGE-7xHA-PEX6-Tomato
FEATURES             Location/Qualifiers
     source          1..12147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             465..491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             495..521
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             540..566
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             570..596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             615..704
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             3648..4346
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(3778..3796)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4046..4065
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4240..4263
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4782..5370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4835..4855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5253..5264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5457..5690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5762..5883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5923..6058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6078..6097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6079..6097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6107..6124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6107..6123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6116..6138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6265..6720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6352..6371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6562..6583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6746..6850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6851..6919,6920..7711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7069..7088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7882..8470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8371..8390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8624..8641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8758..8779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8794..8799,8800..8817,8818..8824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8832..8848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8837..8859
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8856..8872
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8856..8872
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8891..8911
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8893..8911
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        9012..9391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9392..9595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9546..9566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9610..9790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9837..9962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10455..10688
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10873..10917
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11184..11301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11358..12147,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcttg ggctgcaggt cgagatccac cggtcgccac catgtaccct tatgacgtac
      481 ctgactatgc ggggtatccc tatgacgtgc cagactatgc ctcaggagga gggtccggat
      541 atccatacga cgtccccgac tacgcaggtt atccctatga tgtaccagat tacgcgtccg
      601 gttctgctgc tagataccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc
      661 cggactatgc aggatcctat ccatatgacg ttccagatta cgcttctagc gcgctggctg
      721 tcttgcgggt cctggagccc tttccgaccg agacaccccc gttggcagtg ctgctgccac
      781 ccgggggccc gtggccggcg gcggagctgg gcctggtgct ggccctgagg cctgcagggg
      841 agagcccggc agggccggcg ctgctggtgg cagccctgga ggggccggac gcgggcaccg
      901 aagagcaggg tcccgggccg ccgcagctac tggttagccg cgcgctgctg cggctcctgg
      961 cactgggctc cggggcctgg gtgcgggcgc gggcggtgcg gcggcccccg gcgctaggtt
     1021 gggcactgct tggcacctcg ctggggcctg ggctcggacc gcgagtcggg ccgctgctgg
     1081 tgaggcgcgg agagaccctc ccagttcccg gaccgcgggt gctggagacg cggccggcgt
     1141 tgcaagggct gctgggccca gggactcggc tggctgtgac tgagctccgc gggcgggcca
     1201 gactgtgtcc agagtctggg gacagcagtc ggcccccacc cccgcccgtg gtgtcctcct
     1261 ttgcggtttc tggcacagtg cggcgactcc agggagttct gggagggact ggagattcac
     1321 taggggtgag ccggagctgt ctccgtggcc ttggcctctt ccagggcgaa tgggtgtggg
     1381 tggcccaggc cagagagtca tcgaacactt cacagccgca cttggctagg gtgcaggtcc
     1441 tagaacctcg ctgggacctc tctgatagac tgggacccgg ctctggaccg ctgggagagc
     1501 ccctcgctga cggactggcg cttgtccctg ccactttggc ttttaatctt ggctgtgacc
     1561 ccctggaaat gggagagctc agaattcaga ggtacttgga aggctccatc gcccctgaag
     1621 acaaaggaag ctgctcattg ctgcctgggc ctccatttgc cagagagtta cacatcgaaa
     1681 ttgtgtcttc tccccactac agcactaatg gaaattatga cggtgttctt taccggcact
     1741 ttcagatacc cagggtagtc caggaagggg atgttctatg tgtgccaaca attgggcaag
     1801 tagagatcct ggaaggaagt ccagagaaac tgcccaggtg gcgggaaatg ttttttaaag
     1861 tgaagaaaac agttggggaa gctccagatg gaccagccag tgcctacttg gccgacacca
     1921 cccatacctc cttgtacatg gtgggttcta ccctgagccc tgttccatgg ctcccttcag
     1981 aggaatccac tctctggagc agtttgtctc ctccaggcct ggaggccttg gtgtctgaac
     2041 tctgtgctgt cctgaagcct cgcctccagc cagggggtgc cctgctgaca ggaactagca
     2101 gtgtccttct acggggcccc ccaggctgtg ggaagaccac agtagttgct gctgcctgta
     2161 gtcaccttgg gctccactta ctgaaggtgc cctgctccag cctctgtgca gaaagtagtg
     2221 gggctgtgga gacaaaactg caggccatct tctcccgggc ccgccgttgc cggcctgcag
     2281 tcctgttgct cacagctgtg gaccttctgg gccgggaccg tgatgggctg ggtgaggatg
     2341 cccgtgtgat ggctgtgctg cgtcacctcc tcctcaatga ggaccccctc aacagctgcc
     2401 ctcccctcat ggttgtggcc accacaagcc gggcccagga cctgcctgct gatgtgcaga
     2461 cagcatttcc tcatgagctc gaggtgcctg ctctgtcaga ggggcagcgg ctcagcatcc
     2521 tgcgggccct cactgcccac cttcccctgg gccaggaggt gaacttggca cagctagcac
     2581 ggcggtgtgc aggctttgtg gtaggggatc tctatgccct tctgacccac agcagccggg
     2641 cagcctgcac caggatcaag aactcaggtt tggcaggtgg cttgactgag gaggatgagg
     2701 gggagctgtg tgctgccggc tttcctctcc tggctgagga ctttgggcag gcactggagc
     2761 aactgcagac agctcactcc caggccgttg gagcccccaa gatcccctca gtgtcctggc
     2821 atgatgtggg tgggctgcag gaggtgaaga aggagatcct ggagaccatt cagctccccc
     2881 tggagcaccc tgagctactg agcctgggcc tgagacgctc aggccttctg ctccatgggc
     2941 cccctggcac cggcaagacc cttctggcca aggcagtagc cactgagtgc agccttacct
     3001 tcctcagcgt gaaggggcca gagctcatta acatgtatgt gggccaaagt gaggagaatg
     3061 tgcgggaagt gtttgccagg gccagggctg cagctccatg cattatcttc tttgatgaac
     3121 tggactcttt ggccccaagc cgggggcgaa gtggagattc tggaggagtg atggacaggg
     3181 tggtgtctca gctccttgcc gagctagatg ggctgcacag cactcaggat gtgtttgtga
     3241 ttggagccac caacagacca gatctcctgg accctgccct tctgcggcct ggcagatttg
     3301 acaagctggt gtttgtggag gcaaatgagg accgggcctc ccagctacgc gttctaagtg
     3361 ccatcacacg caaattcaag ctagagccat ctgtgagcct ggtaaacgtg ctagattgct
     3421 gccctcccca gctgacgggc gcggacctct actctctctg ctctgatgct atgacagctg
     3481 ccctcaaacg cagggttcat gacctggagg aagggctgga accaggtagc tcagcactga
     3541 tgctcaccat ggaggacttg ctgcaggctg ccgcccggct gcaaccctca gtcagtgagc
     3601 aggagctgct ccggtacaag cgcatccagc gcaagtttgc tgcctgcgtg agcaagggcg
     3661 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
     3721 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     3781 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     3841 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     3901 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     3961 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
     4021 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     4081 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     4141 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     4201 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     4261 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     4321 tgtacggcat ggacgagctg tacaagtaag agccccccag ggtctgggac cccgctcagc
     4381 atggctgcag gtaccttgat agcccacaga gagatctggg aaggaagggc tcctcctcag
     4441 gctgctgcca acccacctgg aggccacctc cctccaggag atcccagggt gcaaagtggc
     4501 attgagacag cagcaacagc tcaagagata tctcctgcct acttgcccct ccttccaggc
     4561 cggctctaag agaaaggccc atctactcag gaagagggcc agggccttgg gttctgggga
     4621 ttgggccctg agagggctag ttctgtggct gaaaataaag catgtcccgc cccctactgg
     4681 tgttgtggca tgaaaggttg gagtgagaaa gagcagggtt gtgggaggag taagccctgg
     4741 gagagggtgg gggggtgggc agcatgagcg gccgcgtcga caatcaacct ctggattaca
     4801 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4861 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4921 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4981 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5041 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5101 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5161 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5221 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5281 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5341 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     5401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5461 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5521 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5581 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5641 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5701 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5761 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     5941 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6001 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6061 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6121 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6181 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6241 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6301 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6361 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6421 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6481 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6541 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6601 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6661 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6841 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6901 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6961 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7021 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7081 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7141 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7201 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7261 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7321 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7381 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7441 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7501 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7561 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7621 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7681 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7741 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7801 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7861 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7921 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7981 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8041 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8101 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8161 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8221 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8281 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8341 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8401 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8461 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8521 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8581 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8641 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8701 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     8761 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     8821 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     8881 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     8941 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     9001 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     9061 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     9421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     9481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     9541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     9601 agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9781 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9841 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9901 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9961 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
    10021 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
    10081 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
    10141 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
    10201 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
    10261 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
    10321 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    10381 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    10441 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    10501 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    10561 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    10621 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    10681 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    10741 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    10801 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    10861 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10921 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10981 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    11041 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    11101 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    11161 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
    11221 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    11281 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagaagct tgattaaccc
    11341 gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
    11401 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
    11461 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
    11521 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
    11581 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
    11641 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
    11701 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
    11761 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
    11821 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
    11881 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
    11941 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
    12001 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
    12061 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
    12121 gttgcggggg cggcagttat ggcggtg
//
