LOCUS       sequence-36132-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO V5 DNAJB4
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     61..85
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             188..229
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
       61 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      121 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttgaatt
      181 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgg gatccaccat
      241 ggggaaagac tattattgca ttttgggaat tgagaaagga gcttcagatg aagatattaa
      301 aaaggcttac cgaaaacaag ccctcaaatt tcatccggac aagaacaaat ctcctcaggc
      361 agaggaaaaa tttaaagagg tcgcagaagc ttatgaagta ttgagtgatc ctaaaaagag
      421 agaaatatat gatcagtttg gggaggaagg gttgaaagga ggagcaggag gtactgatgg
      481 acaaggaggt accttccggt acacctttca tggcgatcct catgctacat ttgctgcatt
      541 tttcggaggg tccaacccct ttgaaatttt ctttggaaga cgaatgggtg gtggtagaga
      601 ttctgaagaa atggaaatag atggtgatcc ttttagtgcc tttggtttca gcatgaatgg
      661 atatccaaga gacaggaatt ctgtggggcc atcccgcctc aaacaagatc ctccagttat
      721 tcatgaactt agagtatcac ttgaagagat atatagtggt tgtaccaaac ggatgaagat
      781 ttctcgaaaa aggctaaacg ctgatggaag gagttacaga tctgaggaca aaattcttac
      841 cattgagatt aaaaaagggt ggaa
//
