LOCUS       sequence-361322-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TC10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     318..334
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     318..334
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     339..358
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        339..357
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(532..1722)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1351..1370
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1770..1775,1776..1792,1793..1798))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1925..2513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2005..2024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccagatat ggtctcatga cgcaatcgct acaacttgcg ctggggttag tccgtgggct
       61 tgacagagaa ctggtagaag gcgttggacg gtttcaagag cctgtttgcc accgtcatga
      121 ctagcaatcg ctacaacttg ctcgggcgtc aggccgtggg cttgacagag aactggtaga
      181 aggcgctgaa cagtctcaag agcctgtttg ccacccccat tagagcgaga ccagctttgg
      241 tctagaggag gcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
      301 acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga
      361 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
      421 tgttcttccc cgatcaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg
      481 ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga
      541 ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc
      601 gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac
      661 gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg
      721 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
      781 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc
      841 cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc
      901 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
      961 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
     1021 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     1081 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     1141 caagggcatg ggtcggcgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1201 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgtaagg agatggcgcc
     1261 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1321 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1381 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagaa tccacaggac
     1441 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac
     1501 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat
     1561 caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat
     1621 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
     1681 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
     1741 ttagcaattt aactgtgata aactaccgca ttaaagctaa tcgatgataa gctgtcaaac
     1801 atgagaatta attcatgttc tttcctgcgt tatcccctga ttctgtggat aacgcaggaa
     1861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2521 ctttgatctt ttctacgggg tctgacgctc agtggaacga cgcgtaactc acgttaaggg
     2581 attttggtca tgggtggctc
//
