LOCUS       sequence-36133-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Laurenti E, Barde I, Verp S, Offner S, Wilson A, Quenneville S, 
            Wiznerowicz M, Macdonald HR, Trono D, Trumpp A
  TITLE     Inducible gene and shRNA expression in resident hematopoietic stem 
            cells in vivo.
  JOURNAL   Stem Cells. 2010 Aug . 28(8):1390-8.
  PUBMED    20641037
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2010 Aug . 28(8):1390-8."
COMMENT     Sequence Label: pFUT
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(210..228,229..251,252..270,271..293,294..312,313..335,
                     336..354,355..377,378..396,397..419,420..438,439..461,
                     462..480)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     600..616
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     601..617
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             726..737
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
       61 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      121 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
      181 aaaaagcaga tcgatccgac tttcactttt ctctatcact gatagggagt ggtaaactcg
      241 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      301 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      361 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      421 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
      481 gtggtaaact cgagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      541 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtact
      601 cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
      661 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
      721 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
      781 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
      841 gtgc
//
