LOCUS       sequence-361353-        2498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pG10
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(99..687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(179..198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     990..1006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     990..1006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1011..1030
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1011..1029
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1204..2394)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2023..2042
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2442..2447,2448..2464,2465..2470))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gcgttatccc ctgattctgt ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
       61 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
      721 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgaccag
      781 atatggtctc atgacgcaat cgctacaact tgctccggag tcagaccgtg ggcttgacag
      841 agaactggca gtaggcgctg tacggtttca agagcctgtt tgccaccatt gttagagcga
      901 gaccagcttt ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc
      961 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
     1021 cactataggg gatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     1081 agctcgacat actgttcttc cccgatcaag ggttggtttg cgcattcaca gttctccgca
     1141 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     1201 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     1261 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     1321 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     1381 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     1441 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     1501 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     1561 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     1621 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     1681 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     1741 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     1801 gttgaaggct ctcaagggca tgggtcggcg ctctccctta tgcgactcct gcattaggaa
     1861 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgtaa
     1921 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     1981 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     2041 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     2101 aatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     2161 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     2221 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     2281 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     2341 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     2401 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct aatcgatgat
     2461 aagctgtcaa acatgagaat taattcatgt tctttcct
//
