LOCUS       sequence-36136-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Mills E, Pham E, Truong K
  TITLE     Structure based design of a Ca2+-sensitive RhoA protein that 
            controls cell morphology.
  JOURNAL   Cell Calcium. 2010 Oct;48(4):195-201.
  PUBMED    20888042
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium."; date: "2010-10"; volume: "48(4)"; pages: "195-201"
COMMENT     Sequence Label: CaM-RhoA(DP)-Venus
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    23..47
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        63..172
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 aattaatacg actcactata ggggaattgt gagcggataa caattccccg gagttaatcc
       61 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
      121 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
      181 tataccatgg gcctgactag tgagcagatt gcagagttca aagaagcctt ctcattattc
      241 gacaaggatg gggacggcac catcaccaca aaggaacttg gcaccgttat gaggtcgctt
      301 ggacaaaacc caacggaagc agaattgcag gatatgatca atgaagtcga tgctgatggc
      361 aatggaacga tttactttcc tgaatttctt actatgatgg ctagaaaaat gaaggacaca
      421 gacagcgaag aggaaatccg agaagcattc cgtgtttttg acaaggatgg gaacggctac
      481 atcagcgctg ctgaattacg tcacgtcatg acaaacctcg gggagaagtt aacagatgaa
      541 gaagttgatg aaatgataag ggaagcagat atcgatggtg atggccaagt aaactatgaa
      601 gagtttgtac aaatgatgac agcaaaggct agtgctgcca tccggaagaa actggtgatt
      661 gttggtgatg gagcctgtgg aaagacatgc ttgctcatag tcttcagcaa ggaccagttc
      721 ccagaggtgt atgtgcccac agtgtttgag aactatgtgg cagatatcga ggtggatgga
      781 aagcaggtag agttggcttt gtgggacaca gctgggctgg aagattatga tcgcctgagg
      841 cccctctcct acccagatac cgatgttata ctgatgtgtt tttccatcga cagccctgat
      901 agtttagaaa a
//
