LOCUS       sequence-361363-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GG2
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     317..333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     317..333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     338..357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        338..356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(531..1721)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1350..1369
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1769..1774,1775..1791,1792..1797))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1924..2512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2004..2023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatatggtct caaccacttg ctccggagtc agaccgtggg cttgacagag aactggcagt
       61 aggcgctgta cggtttcaag agcctgtttg ccaccattgt tactagcaat cgctacaact
      121 tgctccggag tcagaccgtg ggcttgacag agaactggca gtaggcgctg tacggtttca
      181 agagcctgtt tgccaccatt gttactagca atcgctacaa cctgcgagac cagctttggt
      241 ctagaggagg catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
      301 cacgggccag agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat
      361 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
      421 gttcttcccc gatcaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
      481 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
      541 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
      601 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
      661 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
      721 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
      781 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
      841 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
      901 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      961 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     1021 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     1081 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     1141 aagggcatgg gtcggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     1201 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgtaagga gatggcgccc
     1261 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     1321 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     1381 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg
     1441 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
     1501 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
     1561 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
     1621 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     1681 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     1741 tagcaattta actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca
     1801 tgagaattaa ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa
     1861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2521 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga
     2581 ttttggtcat gggtggctcg acca
//
