LOCUS       sequence-361390-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for CRISPRa, lentiviral expression of gRNA scaffold using activator 
            P65 HSF, contains intact LTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Iaffaldano BJ, Marino MP, Reiser J
  TITLE     CRISPR library screening to develop HEK293-derived cell lines with 
            improved lentiviral vector titers.
  JOURNAL   Front Genome Ed. 2023 Jul 13;5:1218328. doi: 
            10.3389/fgeed.2023.1218328. eCollection 2023.
  PUBMED    37520398
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2023-07-13"; volume: "5:1218328. doi"; pages: " 
            10.3389/fgeed.2023.1218328. eCollection 2023"
COMMENT     Sequence Label: pxpr_502-wt-ltr
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     371..388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    505..526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(541..546,547..564,565..571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    579..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     584..606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     603..619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     603..619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     638..658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        640..658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        686..912
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             913..1093
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1140..1265
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1758..1991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2176..2220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2398..2646
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2398..2418
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2569..2588
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2920..3037
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3086..3596
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3515..3533
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(4061..4085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4135..4155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4171..4713
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             4738..5109
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             5125..5178
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5206..5802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5206..5225)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5702..5722
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5892..6525
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6603..6724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6640..6659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6694..6713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6764..6899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6919..6938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6920..6938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6948..6965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6948..6964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6957..6979)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7106..7561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7193..7212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7403..7424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7587..7691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7692..7760,7761..8552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7910..7929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8723..9094,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      661 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
      721 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      781 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      841 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      901 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      961 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1021 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1081 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1141 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1201 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1261 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1321 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1381 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1441 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1501 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1561 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1621 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1921 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     2041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     2101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     2161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     2221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2341 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccagagag
     2401 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     2461 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     2521 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     2581 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2641 aacaccggag acgaccggtt ttttgcgtct ctgtttaaga gctatgctgc gaatacgagg
     2701 gagcagacga tatggcgtcg ctcctctcca cgagagcata tgggctccgt ggtctcgtac
     2761 accatcaggg tacgtatcag acaccatcag ggtctgctcg tattcgcagc atagcaagtt
     2821 taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttga
     2881 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2941 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3001 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3061 agatccactt tggcgccggc tcgagggggc ccgggttgcg ccttttccaa ggcagccctg
     3121 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     3181 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     3241 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     3301 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct tactagtacc ctcgcagacg
     3361 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     3421 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     3481 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     3541 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     3601 atccaactta agcttgccac catgtcgcgg aggcggcaca gctacgagaa cgatggtgga
     3661 caacctcaca aaaggaggaa gacgtctatg ctagcctcca aaaccatcgt tctttcggtc
     3721 ggcgaggcta ctcgcactct gactgagatc cagtccaccg cagaccgtca gatcttcgaa
     3781 gagaaggtcg ggcctctggt gggtcggctg cgcctcacgg cttcgctccg tcaaaacgga
     3841 gccaagaccg cgtatcgagt caacctaaaa ctggatcagg cggacgtcgt tgattccgga
     3901 cttccgaaag tgcgctacac tcaggtatgg tcgcacgacg tgacaatcgt tgcgaatagc
     3961 accgaggcct cgcgcaaatc gttgtacgat ttgaccaagt ccctcgtcgc gacctcgcag
     4021 gtcgaagatc ttgtcgtcaa ccttgtgccg ctgggccgtt cttcaacttt cttgtacaaa
     4081 gtgggcggag gaggtggaag cggaggagga ggaagcggag gaggaggtag cggacctaag
     4141 aaaaagagga aggtggcggc cgctggtacc ccttcagggc agatcagcaa ccaggccctg
     4201 gctctggccc ctagctccgc tccagtgctg gcccagacta tggtgccctc tagtgctatg
     4261 gtgcctctgg cccagccacc tgctccagcc cctgtgctga ccccaggacc accccagtca
     4321 ctgagcgctc cagtgcccaa gtctacacag gccggcgagg ggactctgag tgaagctctg
     4381 ctgcacctgc agttcgacgc tgatgaggac ctgggagctc tgctggggaa cagcaccgat
     4441 cccggagtgt tcacagatct ggcctccgtg gacaactctg agtttcagca gctgctgaat
     4501 cagggcgtgt ccatgtctca tagtacagcc gaaccaatgc tgatggagta ccccgaagcc
     4561 attacccggc tggtgaccgg cagccagcgg ccccccgacc ccgctccaac tcccctggga
     4621 accagcggcc tgcctaatgg gctgtccgga gatgaagact tctcaagcat cgctgatatg
     4681 gactttagtg ccctgctgtc acagatttcc tctagtgggc agggaggagg tggaagcggc
     4741 ttcagcgtgg acaccagtgc cctgctggac ctgttcagcc cctcggtgac cgtgcccgac
     4801 atgagcctgc ctgaccttga cagcagcctg gccagtatcc aagagctcct gtctccccag
     4861 gagcccccca ggcctcccga ggcagagaac agcagcccgg attcagggaa gcagctggtg
     4921 cactacacag cgcagccgct gttcctgctg gaccccggct ccgtggacac cgggagcaac
     4981 gacctgccgg tgctgtttga gctgggagag ggctcctact tctccgaagg ggacggcttc
     5041 gccgaggacc ccaccatctc cctgctgaca ggctcggagc ctcccaaagc caaggacccc
     5101 actgtctccg atatcggcag tggagagggc agaggaagtc tgctaacatg cggtgacgtc
     5161 gaggagaatc ctggcccagg taccacagat ccaccggagc ttaccatgac cgagtacaag
     5221 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5281 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5341 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5401 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5461 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5521 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5581 gccaccgtcg gcgtttcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5641 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5701 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5761 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccgctagctg aacgcgttaa
     5821 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5881 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5941 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     6001 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     6061 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     6121 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     6181 catggcccga gagctgcatc cggagtactt caagaactgc tgacatcgag cttgctacaa
     6241 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     6301 agccctcaga tgctgcatat aagcagctgc tttttgcttg tactgggtct ctctggttag
     6361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     6541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6721 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6841 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6901 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     6961 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7021 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7081 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7141 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7201 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7261 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7321 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7381 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7441 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7501 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7561 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9061 agcccagctt ggagcgaacg acctacaccg aact
//
