LOCUS       sequence-36140-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondrial localization of EGFP, for real-time imaging of 
            mitochondria in transgenic zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kim MJ, Kang KH, Kim CH, Choi SY
  TITLE     Real-time imaging of mitochondria in transgenic zebrafish expressing
            mitochondrially targeted GFP.
  JOURNAL   Biotechniques. 2008 Sep . 45(3):331-4.
  PUBMED    18778258
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2008 Sep . 45(3):331-4."
COMMENT     Sequence Label: pSCAC-69
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..245
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agncctctgg ngtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
       61 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
      121 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      181 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      241 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc
      301 ggtcgcgtaa cgaggttggc tcgctgggtg atcgtggatc cccgcagaac cggagcggcg
      361 cggacgcgag ctagtcccct cagaagtcca gacatggtaa gcttcagcta gaactcgccg
      421 cagacccggt gaggaagaga gcgaaccggg ccttaaccac cctttatata gccgcctcta
      481 ctgggcgggg attaaccatg acatcatcaa gtccagaatt cccaaagtgc tagaggcggg
      541 gtcttgacag aacattcagc ctgtaagcgt cttgtatact acaactccca gtagcactag
      601 ggcggatgca gctgttgagt gaaacgcgcg agttatcacg aagttagggc gaaaggaagg
      661 gtggcactcc ctagtgcgtc ataagctagc ttgcatgcct gagaatttca gaatgtaatg
      721 atacctttgt tagataaggc tgtttacatc tgatagtgga ccttaagccg acacttaaat
      781 gataaaaacg gcaaagaatt gcaagtttga tttgcattgg aaagggtcgc tggcttttgt
      841 gttacacgcc ccttatttgt gcttgattag atgatccccc tgctcgagcc gggcccaagt
      901 gatctccaaa aaataagtac tttttgactg
//
