LOCUS       sequence-361407-        5846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of V5-tagged mouse neurexophilin-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, 
            Bischofberger J, Scheiffele P
  TITLE     Targeted proteoform mapping uncovers specific Neurexin-3 variants 
            required for dendritic inhibition.
  JOURNAL   Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017.
  PUBMED    35550065
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017."
COMMENT     Sequence Label: pDisplay-V5-Nxph1
FEATURES             Location/Qualifiers
     source          1..5846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     719..778
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             785..826
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1597..1614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1603..1827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1959..2547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2039..2058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2876..2923
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2948..2967
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3155..3949)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3155..3949)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3266..3285)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3876..3895
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        4146..4337
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4294..4314
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(4397..5188,5189..5257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5020..5039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5258..5362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5389..5844
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5476..5495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5686..5707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcat
      721 ggagacagac acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga
      781 ctatggcaaa cctattccga atcctcttct tggtctcgat tctacaagat ccgccaacct
      841 aacaaatggt gggaaatcgg aactactgaa gtcagggagc agcaaatcca cgctaaagca
      901 catatggaca gaaagcagca aagacttgtc tatcagtcgg ctcctctcac agactttccg
      961 tggtaaagaa aatgacacag atttggacct gcgctatgac accccagaac cttattctga
     1021 gcaagacctc tgggactggc tgaggaactc cacagatctt caggagcctc ggcccagggc
     1081 caaaagacgg cccattgtta aaactggcaa gtttaagaaa atgtttggat ggggtgattt
     1141 tcattccaac atcaaaacag tgaagctaaa cctgttgata actgggaaaa ttgtagatca
     1201 cggcaatgga acctttagtg tatatttcag gcataactcc actggtcaag ggaatgtatc
     1261 tgtcagcttg gtgcccccaa caaaaatcgt ggaattcgat ttggcacaac aaaccgtgat
     1321 tgatgctaaa gattccaagt ccttcaactg tcgcattgag tatgagaagg ttgacaaggc
     1381 caccaagaac acactctgca actacgaccc ttcaaaaacc tgttaccagg agcagaccca
     1441 aagtcacgtg tcctggctct gctctaagcc cttcaaggtg atctgtattt acatttcctt
     1501 ttatagtaca gactataaac tagtacagaa ggtatgccct gactacaact atcacagtga
     1561 cacaccttac tttccctccg gatgagtcga gatcagcctc gactgtgcct tctagttgcc
     1621 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1681 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1741 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1801 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     1861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2701 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     2761 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     2821 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     2881 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     2941 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     3001 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     3061 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     3121 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3181 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3241 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3301 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3361 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3421 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     3481 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     3541 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     3601 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     3661 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     3721 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     3781 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     3841 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     3901 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     3961 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     4021 tacttctgga atagctcaga ggccgggcct cggcctctgc ataaataaaa aaaattagtc
     4081 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4141 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4201 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4261 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     4321 ggttctttcc gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt
     4381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     5401 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5461 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5521 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5581 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5641 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5701 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5761 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5821 acaaaatatt aacgcttaca atttac
//
