LOCUS       sequence-361408-       10253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse Nrx3-alpha containing insertion at the 
            alternative splice site 4 together with EGFP protein separated by 2A
            peptide. Contains insertions at alternatively splice sites 1,2,3 and
            6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Nguyen TM, Schreiner D, Xiao L, Traunmuller L, Bornmann C, 
            Scheiffele P
  TITLE     An alternative splicing switch shapes neurexin repertoires in 
            principal neurons versus interneurons in the mouse hippocampus.
  JOURNAL   Elife. 2016 Dec 13;5. doi: 10.7554/eLife.22757.
  PUBMED    27960072
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-12-13"; volume: "5. doi"; pages: " 10.7554/eLife.22757"
COMMENT     Sequence Label: Nrxn3-alpha-AS4+
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1429..1482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     sig_peptide     1507..1566
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6004..6021)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6010..6234
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6366..6954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6446..6465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7283..7330
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7355..7374
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7562..8356)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7562..8356)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7673..7692)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8283..8302
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     enhancer        8553..8744
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8701..8721
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8804..9595,9596..9664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9427..9446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9665..9769)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9796..10251
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9883..9902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10093..10114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatctatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagtccgga ctcagatccg gcagtggaga gggcagagga
     1441 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagaattcgg cttggggata
     1501 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     1561 actggtgact atccatatga tgttccagat tatgctgggg cccagccggc cagatctttc
     1621 atgggccttc ctaaccagtg ggcccgctac ctgcgttggg atgcaagcac gcgcagtgac
     1681 ctgagcttcc agttcaagac caatgtttcc actgggctgc tcctgtattt ggatgatggt
     1741 ggtgtctgtg acttcctctg tctctccctg gtggatggcc gcgttcagct ccgctttagc
     1801 atggactgtg ccgagaccac tgtgctatcc aacaagcagg tgaacgacag cagttggcac
     1861 ttcctcatgg tgagccgtga tcgtgtgcgc actgggctgg tgattgatgg tgagggccag
     1921 tctggagagc tacggcccca gcggccttat atggatgtgg tcagtgattt gttcctcggt
     1981 ggcgtccccg ctgacattcg accttctgcc ctgaccctcg atggagtaca gagcatgcct
     2041 ggctttaagg gattaatgct ggatctcaag tatggcaact cggaacctcg gcttctgggg
     2101 agccagagcg tccagttaga agcagaggga ccctgtggcg agcgtccctg tgaaaatggt
     2161 gggatctgct tcctcttgga tggtcatccc acctgtgact gttctaccac tggctatggt
     2221 ggcacactct gctcagaaga tgtcagtcaa ggtccaggcc tctcccatct tatgatgagt
     2281 gaacaagcac gagaggagaa cgtggccacc ttccgaggct cagagtatct gtgctatgac
     2341 ctgtcccaga accccatcca gagcagcagc gacgaaatca ccctttcctt taagacctgg
     2401 caacgcaatg ggctcatcct ccacactggc aagtcggccg actatgttaa cctggctctg
     2461 aaggatggtg cggtctcctt ggtcattaac ctggggtccg gggcctttga ggccattgtg
     2521 gagccagtga atgggaaatt caacgacaac gcctggcatg atgtcaaagt gacacgcaat
     2581 cttcggcagc actcaggcat cggacacgct atggtgacaa tctctgtgga tggcatccta
     2641 accacaacgg gctacactca agaggactac accatgctgg gctcagatga cttcttctat
     2701 gttggtggaa gcccaagtac cgcagacttg ccaggttcac ctgtgagcaa caacttcatg
     2761 ggctgcctta aagaggttgt ttataagaat aatgacatcc gtctagagct gtctcgcttg
     2821 gcccggattg gagacaccaa gatgaaaatc tatggtgaag ttgtgttcaa gtgtgagaac
     2881 gtggccacac tggatcccat caactttgag accccagagg cttacatcag cttgcccaag
     2941 tggaacacca aacgtatggg ctccatttcc tttgactttc gcaccactga gcccaacggc
     3001 ctgattctct tcactcacgg gaagcctcaa gaaaggaaag atgtccggag ccaaaagaac
     3061 acaaaagttg acttctttgc tgtggaactt cttgatggca acctgtattt gctgcttgac
     3121 atgggctctg gcaccatcaa agtgaaggcc actcagaaga aggccaatga tggggaatgg
     3181 taccacgtgg atattcagcg tgatggcaga tcaggtacca tatctgtgaa cagcaggcgc
     3241 acgccattca ccgccagtgg ggagagtgaa attctcgacc tggaggggga catgtatctg
     3301 ggcgggctgc cagagaaccg agctggcctc atcctcccca cggagctctg gaccgccatg
     3361 ctcaactacg gttacgtggg ctgcatccgc gacctgttca tcgatgggcg cagcaagaac
     3421 atccggcagc tggcggagat gcagaacgca gcaggcgtca agtcctcctg ttcacgcatg
     3481 agcgccaagc agtgtgacag ctacccctgc aagaacaacg cggtgtgcaa ggacggctgg
     3541 aaccgcttca tctgcgactg cactggcacc ggctactggg ggagaacctg cgaaagagag
     3601 gcatctatcc tgagctatga cggcagcatg tacatgaagg tcatcatgcc catggtgatg
     3661 cacacggagg cagaggatgt gtccttccgc ttcatgtcac agcgagccta tgggctactg
     3721 gtagccacaa cctccaggga ttctgcagat acactgcgtc tagagttgga tggtgggcgt
     3781 gtcaagctca tggttaactt agactgtatc aggataaact gtaactccag caaaggacct
     3841 gagaccctgt atgcagggca gaagctcaat gacaatgagt ggcacaccgt ccgggtagtg
     3901 cggagaggaa aaagccttaa gctaacagtg gatgatgatg tggctgaggg gacaatggtg
     3961 ggcgaccaca cccgcctgga attccacaat attgaaactg gtatcatgac tgagaagcgt
     4021 tacatctctg tggtcccctc cagtttcatt ggccatctac agagcctcat gttcaatggc
     4081 ttactgtaca ttgatttgtg caaaaatggc gacatcgact actgtgaact gaaggctcgc
     4141 tttggactga gaaacatcat cgccgaccct gtcactttta agaccaagag cagctacctg
     4201 accctcgcca ccctacaggc ttacacctcc atgcacctct tcttccagtt caagaccact
     4261 tcagctgatg gcttcattct cttcaatagc ggagatggca atgattttat tgcagttgag
     4321 ctggtcaagg ggtacataca ctatgtgttt gatctcggca atggtcccaa tgtgatcaaa
     4381 ggcaacagtg accgccccct gaatgacaac cagtggcaca acgtggtcat caccagggac
     4441 agcagtaaca cccacagtct gaaggtggac accaaggtag tcactcaggt catcaatggt
     4501 gccaaaaatc tggatttgaa aggtgacctc tatatggctg gcttagccca gggcatgtac
     4561 agcaaccttc ccaagcttgt ggcctccagg gatggatttc agggctgcct ggcctctgtg
     4621 gacttgaatg gacgcctgcc tgatctcatc aacgatgctc tccacaggag tggacagatc
     4681 gagcgaggct gtgaagttgc gttgaccaaa gctgacctgc aaggacccag tacaacctgt
     4741 caggaggatt catgcgccaa ccagggggtt tgcatgcagc agtgggaagg tttcacctgt
     4801 gactgctcca tgacatcata ttctggaaac cagtgcaatg accctggtgc aacatacatc
     4861 tttgggaaaa gcggtggcct catcctctat acctggccag caaatgacag acccagcaca
     4921 cgctctgacc gtctcgccgt gggcttcagc actactgtga aggatggtat cttagtacgc
     4981 attgacagcg cccctggact tggcgacttc ctccagcttc acatagaaca agggaaaatt
     5041 ggagttgtct tcaatattgg cacagttgac atctccatca aagaagagag aactcctgtc
     5101 aatgatggca aataccacgt tgtgcgcttc accaggaatg ggggaaatgc tacacttcag
     5161 gtcgacaact ggccagtgaa tgagcactat cctacaggca acactgataa tgaacgcctc
     5221 caaatggtaa aacagaaaat ccccttcaaa tataaccggc ccgtagagga gtggctgcag
     5281 gaaaaaggcc ggcagctaac catcttcaac acccaggcgc aaatagccat cggaggaaag
     5341 gacaaaggac gtctctttca aggtcaactc tctgggctct attatgatgg tttgaaagta
     5401 ctgaacatgg cagctgagaa caaccctaat attaaaatca atggaagtgt ccggctagtg
     5461 ggagaagtcc catcagtctc aggaacaaca cagacaacgt ccatgccacc tgaaatgtct
     5521 accaccgtca tggaaaccac caccaccatg gctacgacca ccacccgaaa gaaccgctct
     5581 acagccagca ttcagcccac gtcagatgat cttgtttcat ctgctgaatg ttcaagtgat
     5641 gatgaagact ttgtcgaatg tgaaccaagt acaggtaggt cagcaaaccc cacggagcca
     5701 ggaatcagac gggttccggg ggcctcagag gtgatccggg agtccagcag tacaacaggg
     5761 atggtcgtcg gcattgtggc tgctgccgcc ctctgcatct tgatcctcct gtacgccatg
     5821 tacaagtaca ggaacaggga cgaggggtcc tatcaagtgg acgagacgag gaactacatc
     5881 agcaactcgg cccagagcaa cggcacgctc atgaaggaga agcaagccag ctccaagagc
     5941 ggccataaga aacagaaaaa caaggacaag gagtattatg tgtaagcggc cgctcgagat
     6001 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6061 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6121 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6181 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6241 aggcggaaag aaccagtggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6781 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7081 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg
     7141 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
     7201 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
     7261 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt
     7321 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     7381 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag
     7441 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg
     7501 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg
     7561 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     7621 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     7681 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     7741 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     7801 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg
     7861 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     7921 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     7981 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     8041 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     8101 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     8161 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     8221 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     8281 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     8341 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag
     8401 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgggcctcgg
     8461 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     8521 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     8581 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     8641 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     8701 accctaactg acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca
     8761 ataaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8821 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8881 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8941 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9001 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9061 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9121 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9181 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9241 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9301 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9361 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9421 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9481 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9541 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9601 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9661 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9721 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9781 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     9841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     9901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     9961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    10141 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    10201 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tac
//
