LOCUS       sequence-361409-       10154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse Nrx3-alpha lacking insertion at the alternative 
            splice site 4 together with EGFP protein separated by 2A peptide. 
            Contains insertions at alternatively splice sites 1,2,3 and 6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Nguyen TM, Schreiner D, Xiao L, Traunmuller L, Bornmann C, 
            Scheiffele P
  TITLE     An alternative splicing switch shapes neurexin repertoires in 
            principal neurons versus interneurons in the mouse hippocampus.
  JOURNAL   Elife. 2016 Dec 13;5. doi: 10.7554/eLife.22757.
  PUBMED    27960072
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-12-13"; volume: "5. doi"; pages: " 10.7554/eLife.22757"
COMMENT     Sequence Label: Nrxn3-alpha-AS4-
FEATURES             Location/Qualifiers
     source          1..10154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1429..1482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     sig_peptide     1507..1566
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5905..5922)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5911..6135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6267..6855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6347..6366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7184..7231
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7256..7275
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7463..8257)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7463..8257)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7574..7593)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8184..8203
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     enhancer        8454..8645
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8602..8622
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8705..9496,9497..9565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9328..9347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9566..9670)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9697..10152
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9784..9803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9994..10015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatctatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagtccgga ctcagatccg gcagtggaga gggcagagga
     1441 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagaattcgg cttggggata
     1501 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     1561 actggtgact atccatatga tgttccagat tatgctgggg cccagccggc cagatctttc
     1621 atgggccttc ctaaccagtg ggcccgctac ctgcgttggg atgcaagcac gcgcagtgac
     1681 ctgagcttcc agttcaagac caatgtttcc actgggctgc tcctgtattt ggatgatggt
     1741 ggtgtctgtg acttcctctg tctctccctg gtggatggcc gcgttcagct ccgctttagc
     1801 atggactgtg ccgagaccac tgtgctatcc aacaagcagg tgaacgacag cagttggcac
     1861 ttcctcatgg tgagccgtga tcgtgtgcgc actgggctgg tgattgatgg tgagggccag
     1921 tctggagagc tacggcccca gcggccttat atggatgtgg tcagtgattt gttcctcggt
     1981 ggcgtccccg ctgacattcg accttctgcc ctgaccctcg atggagtaca gagcatgcct
     2041 ggctttaagg gattaatgct ggatctcaag tatggcaact cggaacctcg gcttctgggg
     2101 agccagagcg tccagttaga agcagaggga ccctgtggcg agcgtccctg tgaaaatggt
     2161 gggatctgct tcctcttgga tggtcatccc acctgtgact gttctaccac tggctatggt
     2221 ggcacactct gctcagaaga tgtcagtcaa ggtccaggcc tctcccatct tatgatgagt
     2281 gaacaagcac gagaggagaa cgtggccacc ttccgaggct cagagtatct gtgctatgac
     2341 ctgtcccaga accccatcca gagcagcagc gacgaaatca ccctttcctt taagacctgg
     2401 caacgcaatg ggctcatcct ccacactggc aagtcggccg actatgttaa cctggctctg
     2461 aaggatggtg cggtctcctt ggtcattaac ctggggtccg gggcctttga ggccattgtg
     2521 gagccagtga atgggaaatt caacgacaac gcctggcatg atgtcaaagt gacacgcaat
     2581 cttcggcagc actcaggcat cggacacgct atggtgacaa tctctgtgga tggcatccta
     2641 accacaacgg gctacactca agaggactac accatgctgg gctcagatga cttcttctat
     2701 gttggtggaa gcccaagtac cgcagacttg ccaggttcac ctgtgagcaa caacttcatg
     2761 ggctgcctta aagaggttgt ttataagaat aatgacatcc gtctagagct gtctcgcttg
     2821 gcccggattg gagacaccaa gatgaaaatc tatggtgaag ttgtgttcaa gtgtgagaac
     2881 gtggccacac tggatcccat caactttgag accccagagg cttacatcag cttgcccaag
     2941 tggaacacca aacgtatggg ctccatttcc tttgactttc gcaccactga gcccaacggc
     3001 ctgattctct tcactcacgg gaagcctcaa gaaaggaaag atgtccggag ccaaaagaac
     3061 acaaaagttg acttctttgc tgtggaactt cttgatggca acctgtattt gctgcttgac
     3121 atgggctctg gcaccatcaa agtgaaggcc actcagaaga aggccaatga tggggaatgg
     3181 taccacgtgg atattcagcg tgatggcaga tcaggtacca tatctgtgaa cagcaggcgc
     3241 acgccattca ccgccagtgg ggagagtgaa attctcgacc tggaggggga catgtatctg
     3301 ggcgggctgc cagagaaccg agctggcctc atcctcccca cggagctctg gaccgccatg
     3361 ctcaactacg gttacgtggg ctgcatccgc gacctgttca tcgatgggcg cagcaagaac
     3421 atccggcagc tggcggagat gcagaacgca gcaggcgtca agtcctcctg ttcacgcatg
     3481 agcgccaagc agtgtgacag ctacccctgc aagaacaacg cggtgtgcaa ggacggctgg
     3541 aaccgcttca tctgcgactg cactggcacc ggctactggg ggagaacctg cgaaagagag
     3601 gcatctatcc tgagctatga cggcagcatg tacatgaagg tcatcatgcc catggtgatg
     3661 cacacggagg cagaggatgt gtccttccgc ttcatgtcac agcgagccta tgggctactg
     3721 gtagccacaa cctccaggga ttctgcagat acactgcgtc tagagttgga tggtgggcgt
     3781 gtcaagctca tggttaactt agactgtatc aggataaact gtaactccag caaaggacct
     3841 gagaccctgt atgcagggca gaagctcaat gacaatgagt ggcacaccgt ccgggtagtg
     3901 cggagaggaa aaagccttaa gctaacagtg gatgatgatg tggctgaggg gacaatggtg
     3961 ggcgaccaca cccgcctgga attccacaat attgaaactg gtatcatgac tgagaagcgt
     4021 tacatctctg tggtcccctc cagtttcatt ggccatctac agagcctcat gttcaatggc
     4081 ttactgtaca ttgatttgtg caaaaatggc gacatcgact actgtgaact gaaggctcgc
     4141 tttggactga gaaacatcat cgccgaccct gtcactttta agaccaagag cagctacctg
     4201 accctcgcca ccctacaggc ttacacctcc atgcacctct tcttccagtt caagaccact
     4261 tcagctgatg gcttcattct cttcaatagc ggagatggca atgattttat tgcagttgag
     4321 ctggtcaagg ggtacataca ctatgtgttt gatctcggca atggtcccaa tgtgatcaaa
     4381 ggcaacagtg accgccccct gaatgacaac cagtggcaca acgtggtcat caccagggac
     4441 agcagtaaca cccacagtct gaaggtggac accaaggtag tcactcaggt catcaatggt
     4501 gccaaaaatc tggatttgaa aggtgacctc tatatggctg gcttagccca gggcatgtac
     4561 agcaaccttc ccaagcttgt ggcctccagg gatggatttc agggctgcct ggcctctgtg
     4621 gacttgaatg gacgcctgcc tgatctcatc aacgatgctc tccacaggag tggacagatc
     4681 gagcgaggct gtgaagttgc gttgaccaaa gctgacctgc aaggacccag tacaacctgt
     4741 caggaggatt catgcgccaa ccagggggtt tgcatgcagc agtgggaagg tttcacctgt
     4801 gactgctcca tgacatcata ttctggaaac cagtgcaatg accctggtgc aacatacatc
     4861 tttgggaaaa gcggtggcct catcctctat acctggccag caaatgacag acccagcaca
     4921 cgctctgacc gtctcgccgt gggcttcagc actactgtga aggatggtat cttagtacgc
     4981 attgacagcg cccctggact tggcgacttc ctccagcttc acatagaaca agggaaaatt
     5041 ggagttgtct tcaatattgg cacagttgac atctccatca aagaagagag aactcctgtc
     5101 aatgatggca aataccacgt tgtgcgcttc accaggaatg ggggaaatgc tacacttcag
     5161 gtcgacaact ggccagtgaa tgagcactat cctacaggcc ggcagctaac catcttcaac
     5221 acccaggcgc aaatagccat cggaggaaag gacaaaggac gtctctttca aggtcaactc
     5281 tctgggctct attatgatgg tttgaaagta ctgaacatgg cagctgagaa caaccctaat
     5341 attaaaatca atggaagtgt ccggctagtg ggagaagtcc catcagtctc aggaacaaca
     5401 cagacaacgt ccatgccacc tgaaatgtct accaccgtca tggaaaccac caccaccatg
     5461 gctacgacca ccacccgaaa gaaccgctct acagccagca ttcagcccac gtcagatgat
     5521 cttgtttcat ctgctgaatg ttcaagtgat gatgaagact ttgtcgaatg tgaaccaagt
     5581 acagcaaacc ccacggagcc aggaatcaga cgggttccgg gggcctcaga ggtgatccgg
     5641 gagtccagca gtacaacagg gatggtcgtc ggcattgtgg ctgctgccgc cctctgcatc
     5701 ttgatcctcc tgtacgccat gtacaagtac aggaacaggg acgaggggtc ctatcaagtg
     5761 gacgagacga ggaactacat cagcaactcg gcccagagca acggcacgct catgaaggag
     5821 aagcaagcca gctccaagag cggccataag aaacagaaaa acaaggacaa ggagtattat
     5881 gtgtaagcgg ccgctcgaga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5941 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6001 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6061 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6121 cggtgggctc tatggcttct gaggcggaaa gaaccagtgg cggtaatacg gttatccaca
     6181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct
     7021 atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
     7081 gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg
     7141 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
     7201 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc
     7261 tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc
     7321 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     7381 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     7441 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     7501 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     7561 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     7621 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     7681 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     7741 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     7801 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     7861 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     7921 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     7981 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     8041 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     8101 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     8161 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     8221 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     8281 cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     8341 agctcagagg ccgggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     8401 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     8461 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     8521 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     8581 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc
     8641 ctcaggactc ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg
     8701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     9721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     9781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     9841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     9901 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     9961 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    10021 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    10081 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    10141 cgcttacaat ttac
//
