LOCUS       sequence-361411-        4401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of V5-tagged mouse FAM19A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, 
            Bischofberger J, Scheiffele P
  TITLE     Targeted proteoform mapping uncovers specific Neurexin-3 variants 
            required for dendritic inhibition.
  JOURNAL   Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017.
  PUBMED    35550065
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017."
COMMENT     Sequence Label: FAM19A1-V5
FEATURES             Location/Qualifiers
     source          1..4401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1023..1064
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    1189..1310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1226..1245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1280..1299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1317..1772)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1454..1475)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1666..1685
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1799..1903
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1905..2262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1905..2096
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1928..1948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2113..2248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2297..3091
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2297..3091
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2351..2370)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2961..2980
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3279..3298)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3323..3370
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3699..4287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4188..4207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 caatggtctc tgcaatgtcc tgggccctgt acttgtggat aagtgcttgt gcgatgctgc
      661 tctgccatgg gtcactccaa cacaccttcc agcagcatca cctgcaccgg ccagaaggag
      721 ggacctgtga agtgatcgcg gcccacaggt gttgtaacaa gaaccgcatc gaggagcggt
      781 cacaaacagt gaagtgttcc tgtttacctg ggaaagtggc tgggacaaca agaaaccgac
      841 cttcctgtgt ggatgcctcc atagtaattg ggaaatggtg gtgtgagatg gagccctgcc
      901 tagaaggaga agaatgtaag acactccctg acaattctgg atggatgtgt gctacaggca
      961 acaagattaa gactacacga attcacccaa gaaccgtcga cggtaccgcg ggcccgggat
     1021 ccggcaaacc tattccgaat cctcttcttg gtctcgattc tacatgagcg gccgcgactc
     1081 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1141 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1201 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1261 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     1321 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     1381 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     1441 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     1501 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     1561 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     1621 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     1681 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     1741 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     1801 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1861 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     1921 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     1981 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2041 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2101 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2161 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2221 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     2281 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2341 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2401 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2461 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2521 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2581 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2641 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2701 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2761 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     2821 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     2881 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     2941 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3001 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3061 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3121 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3181 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3241 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     3301 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     3361 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     3421 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     3481 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     3541 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3601 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3841 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4081 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4321 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4381 aaccgtatta ccgccatgca t
//
