LOCUS       sequence-361417-        6896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus
            fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Wang X, Delle C, Asiminas A, Akther S, Vittani M, Brogger P, Kusk P,
            Vo CT, Radovanovic T, Konno A, Hirai H, Fukuda M, Weikop P, Goldman 
            SA, Nedergaard M, Hirase H
  TITLE     Liver-secreted fluorescent blood plasma markers enable chronic 
            imaging of the microcirculation.
  JOURNAL   Cell Rep Methods. 2022 Sep 21;2(10):100302. doi: 
            10.1016/j.crmeth.2022.100302. eCollection 2022 Oct 24.
  PUBMED    36313804
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-09-21"; volume: "2(10):100302. doi"; pages: "
            10.1016/j.crmeth.2022.100302. eCollection 2022 Oct 24"
COMMENT     Sequence Label: pAAV-P3-Alb-Rosmarinus
FEATURES             Location/Qualifiers
     source          1..6896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     misc_feature    3109..3697
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3162..3182)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3580..3591)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3715..3939
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3984..4124
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3984..4113
                     /label=AAV2 ITR
     rep_origin      4199..4654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4286..4305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4496..4517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4671..4690)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4790..4812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4850..4868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4936..5040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5041..5109,5110..5901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5259..5278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6072..6660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6561..6580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctactcg agaccgggcg
      181 cgccggggga ggctgctggt gaatattaac caaggtcacc ccagttatcg gaggagcaaa
      241 caggggctaa gtccacacgc gtggtaccgt ctgtctgcac atttcgtaga gcgagtgttc
      301 cgatactcta atctccctag gcaaggttca tatttgtgta ggttacttat tctccttttg
      361 ttgactaagt caataatcag aatcagcagg tttggagtca gcttggcagg gatcagcagc
      421 ctgggttgga aggagggggt ataaaagccc cttcaccagg agaagccgtc acacagatcc
      481 acaagctcct ggtcgacact agtaccggtg ccaccatgaa gtgggtaacc tttctcctcc
      541 tcctcttcgt ctccggctct gctttttcca ggggtgtgtt tcgccgagaa gcacacaaga
      601 gtgagatcgc ccatcggtat aatgatttgg gagaacaaca tttcaaaggc ctagtcctga
      661 ttgccttttc ccagtatctc cagaaatgct catacgatga gcatgccaaa ttagtgcagg
      721 aagtaacaga ctttgcaaag acgtgtgttg ccgatgagtc tgccgccaac tgtgacaaat
      781 cccttcacac tctttttgga gataagttgt gtgccattcc aaacctccgt gaaaactatg
      841 gtgaactggc tgactgctgt acaaaacaag agcccgaaag aaacgaatgt ttcctgcaac
      901 acaaagatga caaccccagc ctgccaccat ttgaaaggcc agaggctgag gccatgtgca
      961 cctcctttaa ggaaaaccca accaccttta tgggacacta tttgcatgaa gttgccagaa
     1021 gacatcctta tttctatgcc ccagaacttc tttactatgc tgagcagtac aatgagattc
     1081 tgacccagtg ttgtgcagag gctgacaagg aaagctgcct gaccccgaag cttgatggtg
     1141 tgaaggagaa agcattggtc tcatctgtcc gtcagagaat gaagtgctcc agtatgcaga
     1201 agtttggaga gagagctttt aaagcatggg cagtagctcg tctgagccag acattcccca
     1261 atgctgactt tgcagaaatc accaaattgg caacagacct gaccaaagtc aacaaggagt
     1321 gctgccatgg tgacctgctg gaatgcgcag atgacagggc ggaacttgcc aagtacatgt
     1381 gtgaaaacca ggcgactatc tccagcaaac tgcagacttg ctgcgataaa ccactgttga
     1441 agaaagccca ctgtcttagt gaggtggagc atgacaccat gcctgctgat ctgcctgcca
     1501 ttgctgctga ttttgttgag gaccaggaag tgtgcaagaa ctatgctgag gccaaggatg
     1561 tcttcctggg cacgttcttg tatgaatatt caagaagaca ccctgattac tctgtatccc
     1621 tgttgctgag acttgctaag aaatatgaag ccactctgga aaagtgctgc gctgaagcca
     1681 atcctcccgc atgctacggc acagtgcttg ctgaatttca gcctcttgta gaagagccta
     1741 agaacttggt caaaaccaac tgtgatcttt acgagaagct tggagaatat ggattccaaa
     1801 atgccattct agttcgctac acccagaaag cacctcaggt gtcaacccca actctcgtgg
     1861 aggctgcaag aaacctagga agagtgggca ccaagtgttg tacacttcct gaagatcaga
     1921 gactgccttg tgtggaagac tatctgtctg caatcctgaa ccgtgtgtgt ctgctgcatg
     1981 agaagacccc agtgagtgag catgttacca agtgctgtag tggatccctg gtggaaaggc
     2041 ggccatgctt ctctgctctg acagttgatg aaacatatgt ccccaaagag tttaaagctg
     2101 agaccttcac cttccactct gatatctgca cacttccaga gaaggagaag cagattaaga
     2161 aacaaacggc tcttgctgag ctggtgaagc acaagcccaa ggctacagcg gagcaactga
     2221 agactgtcat ggatgacttt gcacagttcc tggatacatg ttgcaaggct gctgacaagg
     2281 acacctgctt ctcgactgag ggtccaaacc ttgtcactag atgcaaagac gccttagccc
     2341 ccgggggtgg aggcggttca ggcggaggtg gctctggcgg tggcggatca gtcgactcag
     2401 tgataaagag cgtcatgaaa attaagctgc acatggacgg catcgtgaac ggtcacaaat
     2461 tcatgatcac aggtgagggg gaaggcaagc catttgaggg gactcatacc attatcctga
     2521 aggtgaagga aggaggacca ctgcctttcg cttatgatat cctcactaca gcctttcagt
     2581 acggcaatcg ggtcttcaca aaatatccca aggatattcc tgactacttc aaacagtcat
     2641 tcccggaggg ctacagttgg gagcggtcca tgacctttga ggatcagggc gtgtgtaccg
     2701 tgacctctga catcaaactg gaaggagact atttcaccta cgatatcagg tttcacggag
     2761 tcaatttccc cgccggcggt cccgtcatgc agaaaaagac tctgcggtgg gaaccaagca
     2821 ccgagaacat gtacgtgcga gacggcgtgc tggtaggcga ggtggagaga actctgctgc
     2881 tggagggcaa taaacatcac cgctgtaact tcagaacaac ttacaaagca aagaaagaag
     2941 tagtcctgcc agaataccac tttgttgacc accggataga gatcttgggg catgacaagg
     3001 actataacaa tgtggttgtg tatgaaaacg cagtggctcg gcaacaggcc tctaccctgc
     3061 caagcaaggc gaagtagcgg ccgcgaattc gatatcaagc ttatcgataa tcaacctctg
     3121 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3181 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3241 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3301 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3361 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3421 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3481 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3541 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3601 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3661 cagacgagtc ggatctccct ttgggccgcc tccccgctct cgagatacgt acgactgtgc
     3721 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3781 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3841 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3901 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagatcta
     3961 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     4021 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4081 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     4141 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     4561 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4681 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4741 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4801 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4861 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4921 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4981 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5041 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5101 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5161 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5221 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5281 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5341 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5401 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5461 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5521 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5581 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5641 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5701 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
     5761 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5821 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5881 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5941 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6001 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6781 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6841 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcag
//
