LOCUS       sequence-361418-       11101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination. May not contain 
            entire coding region of gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11101)
  TITLE     SGC Structures-2021-Q3 and Q4
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LIPS:SDC101a-A08:C34411
FEATURES             Location/Qualifiers
     source          1..11101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(293..310)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1527..1543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1527..1543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1540..1562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1551..1567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1575..1581,1582..1599,1600..1605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1620..1641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1758..1775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1929..2517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2009..2028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2688..3479,3480..3548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3311..3330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3549..3653)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3721..3739
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3777..3799)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3899..3918
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4112..4134
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4126..4143
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4127..4143
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     7316..8143
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             7316..7440
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(7478..8083)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        8147..8238
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     8163..8180
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     8289..8351
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     CDS             9726..9749
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_recomb     10255..10960
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           10343..10715
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(10372..10960)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
ORIGIN
        1 gatgataaat ctaccatcgg tggaggcgca ggcggggctg gcggcggagg cggaggcgga
       61 ggtggtggcg gtgatgcaga cggcggttta ggctcaaatg tctctttagg caacacagtc
      121 ggcacctcaa ctattgtact ggtttcgggc gccgtttttg gtttgaccgg tctgagacga
      181 gtgcgatttt tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca
      241 gcgggttgag gttccgtcgg cattggtgga gcgggcggca attcagacat cgatggtggt
      301 ggtggtggtg gaggcgctgg aatgttaggc acgggagaag gtggtggcgg cggtgccgcc
      361 ggtataattt gttctggttt agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc
      421 gctggctgca caacggaagg tcgtctgctt cgaggcagcg cttggggtgg tggcaattca
      481 atattataat tggaatacaa atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg
      541 tttaccgtgc cgatatttaa caaccgctca atgtaagcaa ttgtattgta aagagattgt
      601 ctcaagctcg cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag
      661 acacttcgct gtcgtcgacg tacatgtatg ctttgttgtc aaaaacgtcg ttggcaagct
      721 ttaaaatatt taaaagaaca tctctgttca gcaccactgt gttgtcgtaa atgttgtttt
      781 tgataatttg cgcttccgca gtatcgacac gttcaaaaaa ttgatgcgca tcaattttgt
      841 tgttcctatt attgaataaa taagattgta cagattcata tctacgattc gtcatggcca
      901 ccacaaatgc tacgctgcaa acgctggtac aattttacga aaactgcaaa aacgtcaaaa
      961 ctcggtataa aataatcaac gggcgctttg gcaaaatatc tattttatcg cacaagccca
     1021 ctagcaaatt gtatttgcag aaaacaattt cggcgcacaa ttttaacgct gacgaaataa
     1081 aagttcacca gttaatgagc gaccacccaa attttataaa aatctatttt aatcacggtt
     1141 ccatcaacaa ccaagtgatc gtgatggact acattgactg tcccgattta tttgaaacac
     1201 tacaaattaa aggcgagctt tcgtaccaac ttgttagcaa tattattaga cagctgtgtg
     1261 aagcgctcaa cgatttgcac aagcacaatt tcatacacaa cgacataaaa ctcgaaaatg
     1321 tcttatattt cgaagcactt gatcgcgtgt atgtttgcga ttacggattg tgcaaacacg
     1381 aaaactcact tagcgtgcac gacggcacgt tggagtattt tagtccggaa aaaattcgac
     1441 accacaacta tgcacgttcg tttgactggt acgccgtcgg cgtgttaaca tacaagttgc
     1501 taaccggcgg ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3661 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3721 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     3781 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     3841 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     3901 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     3961 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     4021 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4081 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtgccaagc ttggctcgta aagcgagttg aaggatcata tttagttgcg tttatgagat
     4201 aagattgaaa gcacgtgtaa aatgtttccc gcgcgttggc acaactattt acaatgcggc
     4261 caagttataa aagattctaa tctgatatgt tttaaaacac ctttgcggcc cgagttgttt
     4321 gcgtacgtga ctagcgaaga agatatgtgg accgcagaac agatagtaaa acaaaaccct
     4381 agtattggag caataatcga tttaaccaac acgtctaaat attatgatgg tgtgcatttt
     4441 ttgcgggcgg gcctgttata caaaaaaatt caagtacctg gccagacttt gccgcctgaa
     4501 agcatagttc aagaatttat tgacacggta aaagaattta cagaaaagtg tcccggcatg
     4561 ttggtgggcg tgcactgcac acacggtatt aatcgcaccg gttacatggt gtgcagatat
     4621 ttaatgcaca ccctgggtat tgcgccgcag gaagccatag atagattcga aaaagccaga
     4681 ggtcacaaaa ttgaaagaca aaattacgtt caagatttat taatttaatt aatattattt
     4741 gcattcttta acaaatactt tatcctattt tcaaattgtt gcgcttcttc cagcgaacca
     4801 aaactatgct tcgcttgctc cgtttagctt gtagccgatc agtggcgttg ttccaatcga
     4861 cggtaggatt aggccggata ttctccacca caatgttggc aacgttgatg ttacgtttat
     4921 gcttttggtt ttccacgtac gtcttttggc cggtaatagc cgtaaacgta gtgccgtcgc
     4981 gcgtcacgca caacaccgga tgtttgcgct tgtccgcggg gtattgaacc gcgcgatccg
     5041 acaaatccac cactttggca actaaatcgg tgacctgcgc gtcttttttc tgcattattt
     5101 cgtctttctt ttgcatggtt tcctggaagc cggtgtacat gcggtttaga tcagtcatga
     5161 cgcgcgtgac ctgcaaatct ttggcctcga tctgcttgtc cttgatggca acgatgcgtt
     5221 caataaactc ttgtttttta acaagttcct cggttttttg cgccaccacc gcttgcagcg
     5281 cgtttgtgtg ctcggtgaat gtcgcaatca gcttagtcac caactgtttg ctctcctcct
     5341 cccgttgttt gatcgcggga tcgtacttgc cggtgcagag cacttgagga attacttctt
     5401 ctaaaagcca ttcttgtaat tctatggcgt aaggcaattt ggacttcata atcagctgaa
     5461 tcacgccgga tttagtaatg agcactgtat gcggctgcaa atacagcggg tcgccccttt
     5521 tcacgacgct gttagaggta gggcccccat tttggatggt ctgctcaaat aacgatttgt
     5581 atttattgtc tacatgaaca cgtatagctt tatcacaaac tgtatatttt aaactgttag
     5641 cgacgtcctt ggccacgaac cggacctgtt ggtcgcgctc tagcacgtac cgcaggttga
     5701 acgtatcttc tccaaattta aattctccaa ttttaacgcg agccattttg atacacgtgt
     5761 gtcgattttg caacaactat tgttttttaa cgcaaactaa acttattgtg gtaagcaata
     5821 attaaatatg ggggaacatg cgccgctaca acactcgtcg ttatgaacgc agacggcgcc
     5881 ggtctcggcg caagcggcta aaacgtgttg cgcgttcaac gcggcaaaca tcgcaaaagc
     5941 caatagtaca gttttgattt gcatattaac ggcgattttt taaattatct tatttaataa
     6001 atagttatga cgcctacaac tccccgcccg cgttgactcg ctgcacctcg agcagttcgt
     6061 tgacgccttc ctccgtgtgg ccgaacacgt cgagcgggtg gtcgatgacc agcggcgtgc
     6121 cgcacgcgac gcacaagtat ctgtacaccg aatgatcgtc gggcgaaggc acgtcggcct
     6181 ccaagtggca atattggcaa attcgaaaat atatacagtt gggttgtttg cgcatatcta
     6241 tcgtggcgtt gggcatgtac gtccgaacgt tgatttgcat gcaagccgaa attaaatcat
     6301 tgcgattagt gcgattaaaa cgttgtacat cctcgctttt aatcatgccg tcgattaaat
     6361 cgcgcaatcg agtcaagtga tcaaagtgtg gaataatgtt ttctttgtat tcccgagtca
     6421 agcgcagcgc gtattttaac aaactagcca tcttgtaagt tagtttcatt taatgcaact
     6481 ttatccaata atatattatg tatcgcacgt caagaattaa caatgcgccc gttgtcgcat
     6541 ctcaacacga ctatgataga gatcaaataa agcgcgaatt aaatagcttg cgacgcaacg
     6601 tgcacgatct gtgcacgcgt tccggcacga gctttgattg taataagttt ttacgaagcg
     6661 atgacatgac ccccgtagtg acaacgatca cgcccaaaag aactgccgac tacaaaatta
     6721 ccgagtatgt cggtgacgtt aaaactatta agccatccaa tcgaccgtta gtcgaatcag
     6781 gaccgctggt gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt gtggagtccg
     6841 ctcattagag cgtcatgttt agacaagaaa gctacatatt taattgatcc cgatgatttt
     6901 attgataaat tgaccctaac tccatacacg gtattctaca atggcggggt tttggtcaaa
     6961 atttccggac tgcgattgta catgctgtta acggctccgc ccactattaa tgaaattaaa
     7021 aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga
     7081 aagaaaaatg tcgtcgacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa
     7141 atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt
     7201 atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta
     7261 atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat
     7321 cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtc
     7381 gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga
     7441 ataataaaac aattataaat gtcaaatttg ttttttatta acgatacaaa ccaaacgcaa
     7501 caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat
     7561 atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt ttattttcac
     7621 ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct
     7681 cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt
     7741 ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag
     7801 tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa
     7861 ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg
     7921 catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat
     7981 aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac
     8041 tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa
     8101 actaatatca caaactggaa atgtctatca atatatagtt gctgatatca tggagataat
     8161 taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta
     8221 ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatca
     8281 gatctaaaat gaaattctta gtcaacgttg cccttgtttt tatggtcgta tacatttctt
     8341 acatctatgc ggccgctaaa gaggtctgct acggacaact tggctgcttt tctgatgaaa
     8401 aaccatgggc aggaaccctt cagcgacctg taaaattact tccctggtcc cccgaggaca
     8461 ttgacacccg ctttcttctg tacacaaatg aaaatccaaa caacttccaa ctaatcactg
     8521 gcacggaacc agacaccatt gaggcttcaa acttccaact ggaccgcaag acacgcttca
     8581 tcatccatgg cttcttagac aaggcggagg acagctggcc atcggacatg tgcaagaaaa
     8641 tgtttgaagt ggagaaggtg aactgcatct gtgtggactg gaggcacggg tcccgggcaa
     8701 tgtacaccca agccgtgcaa aacattcggg ttgttggggc ggagacagct ttcttaatac
     8761 aagcactgtc gacgcagctg gggtacagcc ttgaggacgt gcatgtcatc ggccacagcc
     8821 tgggcgcgca cacggccgcg gaggcgggca ggaggctggg gggccgcgtg ggcaggatca
     8881 cagggctgga tccagcaggg ccgtgcttcc aggatgaacc tgaggaggtt cggttggatc
     8941 catctgacgc cgtgtttgtg gatgtgattc acacagattc ttctcccata gttccttccc
     9001 taggtttcgg aatgagccaa aaggtgggcc atctggattt ctttccaaat ggaggaaagg
     9061 aaatgcccgg atgtaagaaa aatgtccttt caaccattac tgatattgat ggaatatggg
     9121 aaggaattgg tggctttgtg tcttgcaatc acctaagaag cttcgagtat tactcaagca
     9181 gcgtcctcaa ccctgatggc ttcctgggct atccctgtgc ctcctacgat gagtttcagg
     9241 agagtaagtg tttcccttgt ccagctgaag gatgccccaa aatggggcac tatgctgacc
     9301 aatttaaggg gaaaacaagt gctgtggaac aaaccttttt cctgaacaca ggagagagtg
     9361 gtaactttac tagttggaga tataaggtat cagtcacact ttctggaaaa gagaaagtga
     9421 atgggtacat caggattgct ttgtatggaa gtaatgaaaa ctcgaaacaa tatgagattt
     9481 tcaaaggatc cctcaaacca gatgcaagtc acacgtgtgc tattgatgtg gattttaatg
     9541 ttggaaaaat acagaaagtt aaattcctct ggaacaaacg tgggataaat ctatctgagc
     9601 ccaaactggg ggcttcccaa atcacagtgc aaagtggtga agatgggact gagtataatt
     9661 tttgtagcag cgacactgtg gaagaaaacg tcttgcaatc tctttaccct tgtgaattcg
     9721 tcgagcacca ccaccaccac caccaccact aattgattaa tacctaggtc gacgcgatcc
     9781 tttcctggga cccggcaaga accaaaaact cactctcttc aaggaaatcc gtaatgttaa
     9841 acccgacacg atgaagcttg tcgttggatg gaaaggaaaa gagttctaca gggaaacttg
     9901 gacccgcttc atggaagaca gcttccccat tgttaacgac caagaagtga tggatgtttt
     9961 ccttgttgtc aacatgcgtc ccactagacc caaccgttgt tacaaattcc tggcccaaca
    10021 cgctctgcgt tgcgaccccg actatgtacc tcatgacgtg attaggatcg tcgagccttc
    10081 atgggtgggc agcaacaacg agtaccgcat cagcctggct aagaagggcg gcggctgccc
    10141 aataatgaac cttcactctg agtacaccaa ctcgttcgaa cagttcatcg atcgtgtcat
    10201 ctgggagaac ttctacaagc ccatcgttta catcggtacc gactctgctg aagaggagga
    10261 aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct
    10321 gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct
    10381 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
    10441 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
    10501 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
    10561 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
    10621 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
    10681 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
    10741 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aaagcttgga catatttaac
    10801 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
    10861 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
    10921 cacgtccgcg atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt
    10981 ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga
    11041 tggtgcaggc ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc
    11101 t
//
